Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ hGH HPLC columns incorporate the advantages of high-speed Poros chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three-minute quantitation of human growth factor (hGH).
• Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes • Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins, fusion proteins • Hands-off operation—HPLC method can be automated for streamlined operation • Robust—allows for high-performance purification over hundreds of chromatography cycles
High Speed with High Specificity The combination of CaptureSelect™ hGH affinity ligand and POROS™ 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid titer determination and small-scale purification of hGH.
POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.
Automation POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.
Multiple Cycles Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
For Research Use Only. Not for use in diagnostic procedures.
Spezifikationen
Kapazität (metrisch)
0.1 mL
Matrix
POROS
Max. Elutionsvolumen
29 column volumes
Min. Elutionsvolumen
14 column volumes
Zur Verwendung mit (Geräte)
FPLC
Hochdurchsatz-Kompatibilität
Automated Protocols
Max. Betriebsdruck
2600 psi, 180 bar
Partikelgröße
20 μm
Produktlinie
CaptureSelect™, POROS™
Menge
1 Column
Ziel
Protein
Typ
Column
Stationäre Phase
CaptureSelect™ hGH
Format
Pre-packed Column
Säulentyp
Affinity, Growth Hormone Affinity Ligand, Human Specific
Inhalt und Lagerung
Column includes EZ grip fittings and column test certificate. Store at 2–8°C. DO NOT FREEZE.