eBioscience™ BrdU Staining Kit for Flow Cytometry APC

Katalognummer	Menge
8817-6600-42	100 Tests

Katalognummer 8817-6600-42

Preis (EUR)

982,00

Zum Warenkorb hinzufügen

Menge:

100 Tests

Preis (EUR)

982,00

Zum Warenkorb hinzufügen

Das eBioscience BrdU-Färbekit für die Durchflusszytometrie beinhaltet alle notwendigen Reagenzien und Puffer, um proliferierende Zellen von Säugetieren mittels durchflusszytometrischer Analysen zu identifizieren und zu untersuchen. Die Zykluszellen werden mit 5-Brom-2’-deoxyuridin (BrdU) inkubiert, einem synthetischen Analog von Thymidin, das während der S-Phase der Mitose in frisch synthetisierte genomische DNA integriert wird. Nach der DNA-Denaturierung werden die Zellen für die BrdU-Integrierung zusammen mit allen anderen Zelloberflächen und/oder intrazellulären Zielen von Interesse gefärbt.

Dieses Kit ist optimiert, um eine hellere Färbung gegenüber herkömmlichen Protokollen zu erreichen.

Reaktivität/Spezies
Rind, Hund, Kuh, Mensch, Affe, Maus, nicht-menschlicher Primat, Ratte, Rhesusaffe

Konjugat
APC

Laser
Roter Laser

Emission
660 nm

Anregung
633 bis 647 nm

Berichtete Anwendung
Intrazelluläre Färbung, gefolgt von Durchflusszytometrie-Analyse

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

NachweisverfahrenFluoreszent

FormatKit

Menge100 Tests

Zur Verwendung mit (Anwendung)BrdU Färbung

Zur Verwendung mit (Geräte)Durchflusszytometer

ProduktlinieeBioscience

ProdukttypFärbekit

Unit SizeEach

Häufig gestellte Fragen (FAQ)

Can I also stain for intracellular targets such as transcription factors and cytokines using the eBioscience BrdU staining kit?

Yes, primary antibodies against additional intracellular targets can be added alongside the Anti-BrdU antibody. However, it is critical that you empirically determine whether these additional antibodies are compatible with this staining protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the BrdU in the eBioscience BrdU staining kit supplied as a sterile formulation for in vivo use?

Yes, the BrdU is supplied as a sterile liquid. The vial should be opened under sterile conditions if it will be used in vivo.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I keep the diluted (1 mM) BrdU in the eBioscience BrdU staining kit at 4 degrees C? Can I refreeze it after use?

The stock (32.5 mM) BrdU can be frozen and thawed up to three times. Furthermore, the stock BrdU can be kept at 4 degrees C for up to one week. However, the diluted (1 mM) BrdU should be used immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Have the BrdU Staining kits been tested on proliferating cells of different species?

We have used this kit to detect proliferating human and mouse cells. Other species have yet to be tested but feline, non-human primate and canine should also work.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the stopping points during flow cytometric analysis when using a BrdU staining kit?

Samples can be stored at 4 degrees C for up to 16 hours (i.e., overnight) after fixation with BrdU Staining Buffer (with or without washing in Flow Cytometry Staining Buffer) with no adverse effect on performance.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all eBioscience™ BrdU Staining Kit for Flow Cytometry APC FAQs