Gibco™

BG-11-Medien, optimiert für Cyanobakterien

Name: BG-11-Medien, optimiert für Cyanobakterien
SKU: A1379902
Price: 428.00 EUR

Gibco™ BG-11 Medien sind für die Kultivierung und Pflege ausgewählter Cyanobakterien einschließlich Synechococcus elongates optimiert. Die Formulierung wird gebrauchsfertig inWeitere Informationen

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Katalognummer	Menge
A1379902	6 L
A1379901	1 l

2 Optionen

Katalognummer A1379902

Preis (EUR)

428,00

Each

Menge:

6 L

Preis (EUR)

428,00

Each

Gibco™ BG-11 Medien sind für die Kultivierung und Pflege ausgewählter Cyanobakterien einschließlich Synechococcus elongates optimiert. Die Formulierung wird gebrauchsfertig in einfacher Konzentration geliefert, damit Sie aufwendige Medienvorbereitungsschritte vermeiden können.

Jedes Gibco™ BG-11 Medium wird in der preisgekrönten Gibco™ Flasche geliefert, die für eine einfachere Verwendung im Biosicherheitsschrank konzipiert wurde, das Kontaminationsrisiko minimiert und Ihnen hilft, Zellen konsistenter zu kultivieren. Zusammen ergeben die hochwertige Verpackung und die hohe Qualität, die höhere Zuverlässigkeit und die verbesserte Konsistenz in der Cyanobakterien-Kultur eine bessere Gesamteffizienz und robustere Daten (Abb. 1, 2).

Nur für Forschungszwecke. Nicht für therapeutische oder diagnostische Zwecke bei Menschen und Tieren.

Specifications

Konzentration1X.

MedientypAlgal-Medien

VorbereitungsmethodeKeine (gebrauchsfertig)

Menge6 L

FormFlüssig

ProdukttypMikrobiologische Medien

Klasse des ZielorganismusCyanobakterien

pH6 bis 8

Unit SizeEach

Inhalt und Lagerung

Gibco™ BG-11 Nährmedien enthalten:

• 6 Gibco™ BG-11 Medien – Lagerung bei 4 °C.

Häufig gestellte Fragen (FAQ)

I'm getting low basal expression of my gene of interest when using the pSyn_1 vector. What do you suggest I try?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between pSyn_1 and pSyn_6?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).

The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the shelf-life/competency of Synechococcus?

After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the mechanism of integration when using the Synechococcus expression kits?

A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the strain of Synechococcus used in your kits?

We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all BG-11-Medien, optimiert für Cyanobakterien FAQs