BG-11 Media, optimized for cyanobacteria, 6 L - FAQs

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.

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The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).

The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.

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After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.

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A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.

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We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.

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We offer ready-to-use BG-11 Media, optimized for Cyanobacteria (Cat. No. A1379901). There are several BG11 media formulations. Please see our formulation below.

Our BG-11 media have been qualified for culturing Synechococcus elongatus (strain PCC 7942).
Item, Formula, (%w/v)
Boric Acid, H3BO3, 0.00287%
Manganese Chloride Tetrahydrate, MnCl2-4H2O, 0.00181%
Zinc Sulphate Heptahydrate, ZnSO4-7H2O, 0.00022%
Sodium Molybdate Dihydrate, Na2MoO4, 0.00039%
Copper Sulphate Pentahydrate, CuSO4-5H2O, 0.00008%
Sodium Nitrate, NaNO3, 0.15000%
Calcium Chloride Dihydrate, CaCl2-2H2O, 0.00270%
Ferric Ammonium Citrate (Green), C6H5 4yFexNyO7, 0.00120%
EDTA, EDTA, 0.00010%
Potassium Phosphate Dibasic, K2HPO4, 0.00390%
Magnesium Sulfate Heptahydrate, MgSO4-7H2O, 0.00750%
Sodium Carbonate Monohydrate, Na2CO3-1H2O, 0.00200%

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It includes the pSyn_6 vector. This vector has been tested for protein expression in the Synechococcus elongatus PPC 7942. You can get the Synechococcus elongatus PPC 7942 from academic resources (https://genome.jgi.doe.gov/portal/synel/synel.info.html) or from the ATCC Collection (https://www.atcc.org/products/all/33912.aspx).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.