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Zitierungen und Referenzen (10)

Gibco™

StemFlex™ Medium

Katalognummer	Menge
A3349401	450 mL Basalmedium, 50 mL-Supplement

Katalognummer A3349401

Preis (EUR)

448,65

Online Exclusive

467,00

Ersparnis 18,35 (4%)

Menge:

450 mL Basalmedium, 50 mL-Supplement

Preis (EUR)

448,65

Online Exclusive

467,00

Ersparnis 18,35 (4%)

Gibco™ StemFlex™ Medium unterstützt die robuste Expansion von Feeder-freien pluripotenten Stammzellen (PSCs) und ist für die Unterstützung neuartiger Anwendungen optimiert, einschließlich Single-Cell-Passage, Genbearbeitung und Genreprogrammierung. Die einzigartige Formulierung von StemFlex Medium bietet den Komfort eines flexiblen Fütterungsplans (einschließlich wochenendfreier Optionen) und auch die Möglichkeit, die Matrix und das Passage-Reagenz zu wählen, das am besten für bestimmte Anwendungen geeignet ist. StemFlex Medium ermöglicht die langfristige Feeder-freie Kultur von PSCs ohne karyotypische Anomalien und erhält die Fähigkeit der Zellen, sich in alle drei Keimblätter zu differenzieren.

Hervorragende Ergebnisse
StemFlex Medium ist so formuliert, dass es bei den heutigen anspruchsvolleren Anwendungen, die PSC-Kulturen belasten, wie z. B. Reprogrammierung, Einzelzellpassage und Genbearbeitung, hervorragende Ergebnisse liefert. StemFlex Medium sorgt für ein hervorragendes Zellüberleben im Vergleich zum führenden Mitbewerber (mTeSR1) und ermöglicht so einen größeren Erfolg bei den wichtigsten Zelltypen.

Flexibilität
StemFlex Medium ermöglicht eine beispiellose Flexibilität mit 3-fachem Nutzen für PSC-Kulturen: Möglichkeit zur Auswahl eines optimalen Zuführungsplans, einschließlich einer Option ohne Wochenende, eine Auswahl an Matrizen passend für die Anforderungen des Workflows sowie die Möglichkeit, das Durchflussreagenz an das gewünschte Maß an Zelldissoziation anzupassen.

Hervorragendes Preis-Leistungs-Verhältnis
StemFlex Medium wird in einem praktischen Zweikomponenten-Kit (450 ml Basalmedium und 50 ml Supplement) geliefert. Die Verwendung mit Geltrex™ LDEV-freier Basalmembran-Matrix mit reduziertem Wachstumsfaktor bietet ein kostengünstiges, robustes System für eine hervorragende Zufluss-freie Kultur humaner PSCs. StemFlex Medium ermöglicht eine wochenendfreie Kultur von PSCs und bietet als robustes, Zufluss-freies PSC-Medium zahlreiche Vorteile gegenüber anderen Produkten.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelllinieEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

ZelltypEmbryonale Stammzellen, induzierte pluripotente Stammzellen

KonzentrationStemFlex Basal Medium (1X); StemFlex Supplement (10X)

Fertigungsqualität, HerstellungsqualitätcGMP für medizinische Geräte, 21 CFR Teil 820 und ISO 13485

ProduktlinieStemFlex

ProdukttypStammzell-Medien

Menge450 mL Basalmedium, 50 mL-Supplement

Haltbarkeit24 months From Date of Manufacture

VersandbedingungSupplement auf Trockeneis versenden, das Basal-Medium kann bei Raumtemperatur versendet werden

KlassifikationAnimal Origin

Culture TypeFeeder-freie PSC-Kultur, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

FormLiquid

Serum LevelSerum-free

SterilitätSterile-filtered

Sterilization MethodSterile-filtered

Mit AdditivenPhenolrot

Unit SizeEach

Inhalt und Lagerung

Basalmedium, 2 bis 8°C
Ergänzung, −5 bis −30°C

Häufig gestellte Fragen (FAQ)

You recommend Geltrex matrix as the matrix for routine culture of StemFlex Medium. Can I also use Vitronectin or rhLaminin-521?

Yes. We recommend following the coating instructions for Vitronectin or rhLaminin-521 and using the culture and passaging recommendations in the StemFlex protocol.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A400457 or A400458), Geltrex Flex matrix (Cat. No. A4000046801, A4000046802, or A4000046803), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?

The StemFlex Medium (Cat. No. A3349401) includes 3.151 g/L (~17.5 mM) of D-glucose (dextrose) in the Basal Medium. The StemFlex Medium Supplement does not include glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What media do you recommend for standard single-cell cloning of iPSCs?

We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all StemFlex™ Medium FAQs

Zitierungen und Referenzen (10)

Zitierungen und Referenzen

Abstract

Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.

Authors:Daniszewski M, Nguyen Q, Chy HS, Singh V, Crombie DE, Kulkarni T, Liang HH, Sivakumaran P, Lidgerwood GE, Hernández D, Conquest A, Rooney LA, Chevalier S, Andersen SB, Senabouth A, Vickers JC, Mackey DA, Craig JE, Laslett AL, Hewitt AW, Powell JE, Pébay A

Journal:iScience

PubMed ID:30267684

'We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells ... More

Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.

Authors:Chen YH, Pruett-Miller SM

Journal:Stem Cell Res

PubMed ID:30099335

'The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens. Unfortunately, the procedures for genome editing of hPSCs often subject cells to harsh conditions that compromise viability: a major problem that is compounded by ... More

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.

Authors:Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D

Journal:Cell Rep

PubMed ID:32460021

'CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence of deleterious on-target effects (OnTEs) in human stem cells after insertion of disease-related mutations by ... More

Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells.

Authors:Chen SW, Himeno M, Koui Y, Sugiyama M, Nishitsuji H, Mizokami M, Shimotohno K, Miyajima A, Kido T

Journal:Sci Rep

PubMed ID:32873852

'Hepatocytes derived from human iPSCs are useful to study hepatitis B virus (HBV) infection, however infection efficiency is rather poor. In order to improve the efficiency of HBV infection to iPSC-derived hepatocytes, we set a co-culture of hepatocytes with liver non-parenchymal cells and found that liver sinusoidal endothelial cells (LSECs) ... More

A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.

Authors:Sridharan B, Hubbs C, Llamosas N, Kilinc M, Singhera FU, Willems E, Piper DR, Scampavia L, Rumbaugh G, Spicer TP

Journal:Sci Rep

PubMed ID:31227747

'Neurons created from human induced pluripotent stem cells (hiPSCs) provide the capability of identifying biological mechanisms that underlie brain disorders. IPSC-derived human neurons, or iNs, hold promise for advancing precision medicine through drug screening, though it remains unclear to what extent iNs can support early-stage drug discovery efforts in industrial-scale ... More

View all StemFlex™ Medium citations