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NuPAGE™ MOPS SDS-Laufpuffer (20x)
Zitierungen und Referenzen (1)
Invitrogen™

NuPAGE™ MOPS SDS-Laufpuffer (20x)

NuPAGE MOPS SDS-Laufpuffer (20X) dient dem Lauf von Proteinen auf NuPAGE Bis-Tris-Gelen. Er wird zur Trennung von mittelgroßen bis großenWeitere Informationen
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KatalognummerMenge
NP0001025 l
NP0001500 ml
Katalognummer NP000102
Preis (EUR)
962,00
Each
Menge:
5 l
Preis (EUR)
962,00
Each
NuPAGE MOPS SDS-Laufpuffer (20X) dient dem Lauf von Proteinen auf NuPAGE Bis-Tris-Gelen. Er wird zur Trennung von mittelgroßen bis großen Proteinen empfohlen.

Verwenden Sie den richtigen Puffer zur Optimierung von Proteintrennungen
NuPAGE MES SD-Laufpuffer und NuPAGE MOPS SD-Laufpuffer können beide mit NuPAGE BIS-TRIS-Gelen verwendet werden. MES hat einen niedrigeren pKa-Wert als MOPS, wodurch der MES SD-Laufpuffer schneller ist als der MOPS SD-Laufpuffer. Der Unterschied bei der Ionenmigration wirkt sich auf das Konzentrieren der Proben aus und führt zu einem Unterschied im Proteintrennungsbereich zwischen diesen Puffern. Die Verwendung des MOPS-Puffers ermöglicht eine langsamere Proteinlaufgeschwindigkeit als die Verwendung von MES-Puffer.

Vergleichen Sie Proteinmigrationsmuster mit MES und MOPs auf NuPAGE BIS-TRIS-Gelen

Sehen Sie sich alle verfügbaren Puffer und Reagenzien für SDS-PAGE an

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemischer Name oder MaterialLaufpuffer
Zusammensetzung50 mM MOPS, 50 mM Tris-Base, 0,1 % SDS, 1 mM EDTA, pH 7,7; 0.01-0.09% N,N-dimethylformamide
Empfohlene LagerungInhalt: NuPAGE™ MOPS SDS Laufpuffer (20x)
Lagerung: +4 °C° bis 25 °C°
Konzentration20 X
Physikalische FormFlüssigkeit
ProduktlinieNuPAGE
Menge5 l
pHpH 7,7
Unit SizeEach

Häufig gestellte Fragen (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions?

We do not recommend using NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions. This buffer system may generate excessive heat, resulting in poor band resolution. Further, the protein of interest may not migrate very well in a neutral pH environment if it is not charged.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ MOPS SDS-Laufpuffer (20x) FAQs

Zitierungen und Referenzen (1)

Zitierungen und Referenzen
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More