Zitierungen und Referenzen (6)

Invitrogen™

Hoechst 33342 Ready Flow™ Reagent

Katalognummer	Menge
R37165	120 Assays

Katalognummer R37165

Preis (EUR)

273,00

Menge:

120 Assays

Preis (EUR)

273,00

Das Hoechst 33342 Ready Flow™ Reagenz ist ein heller, anwenderfreundlicher, zelldurchlässiger Farbstoff für die Zellzyklus-Analyse auf einem Durchflusszytometer. Dieser Farbstoff emittiert blaue Fluoreszenz, wenn er an doppelsträngige DNA mit einem Emissionsmaximum von 461 nm gebunden ist.

• Stabil bei Raumtemperatur
• Praktische, gebrauchsfertige Formate—kein Verdünnen, Wiegen oder Pipettieren
• Zelldurchlässig und wirksam bei der Färbung fixierter Zellen und Lebend

Das Hoechst 33342 Ready Flow Reagenz ist ein zelldurchlässiger, blau fluoreszierender DNA-Färbemittel für die Zellzyklus-Analyse. 2 Tropfen aus der Tropfflasche direkt in 1 x 10⁶-Zellen geben, inkubieren und analysieren.

Das Hoechst 33342 Reagenz bindet bevorzugt an Adenin-Thymin-Regionen doppelsträngiger DNA. Dieser Farbstoff bindet sich an die kleine Furche in der DNA und besitzt vom Verhältnis Farbstoff:Basenpaaar abhängige charakteristische Fluoreszenz-Emissionsspektren. Das Hoechst 33342 Ready Flow Reagenz wird durch UV-Licht angeregt und hat Spitzenemissionen bei 460 nm.

Specifications

FarbeBlau

FarbstofftypMembrangängig

Anregungswellenlängenbereich361/497

ProduktlinieReady Flow

Menge120 Assays

ProdukttypNukleinsäurefärbemittel

SubCellular LocalizationNucleus

Unit SizeEach

Inhalt und Lagerung

2 Tropfflaschen, bei Raumtemperatur lagern

Häufig gestellte Fragen (FAQ)

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (6)

Zitierungen und Referenzen

Abstract

Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect.

Authors:Xiang C, Yang K, Liang Z, Wan Y, Cheng Y, Ma D, Zhang H, Hou W, Fu P

Journal:Transl Res

PubMed ID:29324234

'Microvesicles (MVs) are emerging as a new mechanism of intercellular communication by transferring cellular components to target cells, yet their function in disease is just being explored. However, the therapeutic effects of MVs in cartilage injury and degeneration remain unknown. We found MVs contained high levels of sphingosine-1-phosphate (S1P) compared ... More

The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier.

Authors:Robilliard LD, Kho DT, Johnson RH, Anchan A, O'Carroll SJ, Graham ES

Journal:Biosensors (Basel)

PubMed ID:29973526

'In this paper, we demonstrate the application of electrical cell-substrate impedance sensing (ECIS) technology for measuring differences in the formation of a strong and durable endothelial barrier model. In addition, we highlight the capacity of ECIS technology to model the parameters of the physical barrier associated with (I) the paracellular ... More

Diverse Signaling by TGFß Isoforms in Response to Focal Injury is Associated with Either Retinal Regeneration or Reactive Gliosis.

Authors:Conedera FM, Quintela Pousa AM, Presby DM, Mercader N, Enzmann V, Tschopp M

Journal:Cell Mol Neurobiol

PubMed ID:32219603

'Müller cells may have stem cell-like capability as they regenerate photoreceptor loss upon injury in some vertebrates, but not in mammals. Indeed, mammalian Müller cells undergo major cellular and molecular changes summarized as reactive gliosis. Transforming growth factor beta (TGFß) isoforms are multifunctional cytokines that play a central role, both ... More

ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1ß activated endothelium in comparison to TNFa.

Authors:Kho DT, Johnson R, Robilliard L, du Mez E, McIntosh J, O'Carroll SJ, Angel CE, Graham ES

Journal:PLoS One

PubMed ID:28732059

We have previously shown that TNFa and IL-1ß differentially regulate the inflammatory phenotype of human brain endothelial cells (hCMVECs). In this regard, IL-1ß treatment was considerably more potent than TNFa at increasing expression of inflammatory chemokines and leukocyte adhesion molecules. We therefore hypothesised that interaction of the hCMVECs with human ... More

Colloid chemistry pitfall for flow cytometric enumeration of viruses in water.

Authors:Dlusskaya EA, Atrazhev AM, Ashbolt NJ

Journal:Water Res X

PubMed ID:31194069

Flow cytomtery (FCM) has become a standard approach to enumerate viruses in water research. However, the nature of the fluorescent signal in flow cytometric analysis of water samples and the mechanism of its formation, have not been addressed for bacteriophages expected in wastewaters. Here we assess the behaviour of fluorescent ... More

View all Hoechst 33342 Ready Flow™ Reagent citations

Hoechst 33342 Ready Flow™ Reagent

Häufig gestellte Fragen (FAQ)

Is DAPI a good live-cell nuclear label?

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

I would like to label only 100 &micro;L of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

What is the concentration of dye/reagent in the Ready Flow products?

Can I use the ReadyProbes reagents for flow cytometry?

Zitierungen und Referenzen (6)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?