SNARF™-4F 5-(and-6)-Carboxylic Acid
SNARF™-4F 5-(and-6)-Carboxylic Acid
Zitierungen und Referenzen (7)
Invitrogen™

SNARF™-4F 5-(and-6)-Carboxylic Acid

Der zellundurchlässige ratiometrische pH-Indikator SNARF™-4F 5-(und-6)-Carbonsäure (pKa von ∼6,4) weist eine signifikante pH-abhängige Emissionsverschiebung von gelb-orange zur tiefroten Fluoreszenz unterWeitere Informationen
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KatalognummerMenge
S239201 mg
Katalognummer S23920
Preis (EUR)
662,00
Each
Zum Warenkorb hinzufügen
Menge:
1 mg
Preis (EUR)
662,00
Each
Zum Warenkorb hinzufügen
Der zellundurchlässige ratiometrische pH-Indikator SNARF™-4F 5-(und-6)-Carbonsäure (pKa von ∼6,4) weist eine signifikante pH-abhängige Emissionsverschiebung von gelb-orange zur tiefroten Fluoreszenz unter sauren bzw. basischen Bedingungen auf. Diese pH-Wert-Abhängigkeit ermöglicht es, das Verhältnis der Fluoreszenzintensitäten aus dem Farbstoff bei zwei Emissionswellenlängen, typischerweise 580 nm und 640 nm, für quantitative pH-Wert-Bestimmungen zu verwenden.

Weitere Informationen zu Ionenindikatoren wie Calcium-, Kalium-, pH-Wert- und Membranpotentialindikatoren ›
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
NachweisverfahrenFluoreszent
FarbstofftypFarbstoffbasiertes Fluoreszenzmittel
AnzeigepH-Indikator
Menge1 mg
VersandbedingungRaumtemperatur
Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Durchflusszytometer, Mikrotiterplatten-Lesegerät
ProduktlinieSNARF
ProdukttyppH-Indikator
Unit SizeEach
Inhalt und Lagerung
Lagerung bei Raumtemperatur und vor Licht geschützt

Zitierungen und Referenzen (7)

Zitierungen und Referenzen
Abstract
Application of a pH-sensitive fluoroprobe (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms.
Authors:Hunter RC, Beveridge TJ
Journal:Appl Environ Microbiol
PubMed ID:15870340
'An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorhodafluor-4F 5-(and-6) carboxylic acid (C-SNARF-4) ... More
Characterization of dual-wavelength seminaphthofluorescein and seminapthorhodafluor dyes for pH sensing under high hydrostatic pressures.
Authors:Salerno M, Ajimo JJ, Dudley JA, Binzel K, Urayama P
Journal:Anal Biochem
PubMed ID:17274941
'Hydrostatic pressure is an important physical parameter in biology, with pressures in the few-hundred-atm range having significant effects on cellular morphology, metabolism, and viability. To ensure valid results when studying pressure effects using fluorescence spectroscopy and imaging methods, metabolic probes need to be characterized for high-pressure use. Of interest is ... More
Fluorescent indicators for intracellular pH.
Authors:Han J, Burgess K,
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Authors:Liu J, Diwu Z, Leung WY
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission ... More
Single cell kinetics of intracellular, nonviral, nucleic acid delivery vehicle acidification and trafficking.
Authors:Kulkarni RP, Mishra S, Fraser SE, Davis ME,
Journal:Bioconjug Chem
PubMed ID:16029041
Mechanistic understanding of the intracellular trafficking of nonviral nucleic acid delivery vehicles remains elusive. A live, single cell-based assay is described here that is used to investigate and quantitate the spatiotemporal, intracellular pH microenvironment of polymeric-based nucleic acid delivery vehicles. Polycations such as polyethylenimine (PEI), poly-l-lysine (PLL), beta-cyclodextrin-containing polymers lacking ... More
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