iBind™ Western-Starterkit

Katalognummer	Menge
SLF1000S	1 Kit

Katalognummer SLF1000S

Preis (EUR)

1.360,00

1 kit

Zum Warenkorb hinzufügen

Menge:

1 Kit

Preis (EUR)

1.360,00

1 kit

Zum Warenkorb hinzufügen

Das iBind Western-Starterkit enthält ein iBind Western-Gerät sowie alle Verbrauchsmaterialien und Reagenzien, die für den Einstieg mit diesem automatisierten Western-Verarbeitungsgerät erforderlich sind. Das iBind Western-Gerät führt jeden Schritt des Western-Protokolls von der Blockierung über Waschvorgänge bis hin zur Antikörperinkubation über sequenziellen lateralen Fluss (SLF) durch. SLF ermöglicht die zeitnahe Freisetzung und den Fluss von Lösungen und Antikörpern gegen die Membran ohne externe Stromquelle, Schüttler oder Tabletts.

Erfahren Sie mehr über die iBind Western-Systeme ›

Das iBind Western-Gerät funktioniert ausschließlich mit iBind Karten und dem iBind Lösungskit, um eine Minimembran für den Immunnachweis zu verarbeiten. Das iBind Gerät bietet Komfort durch Automatisierung und hervorragende Reproduzierbarkeit gegenüber herkömmlichen manuellen Methoden. Jede Lösung wird über SLF in ∼2,5 Stunden aus den iBind Geräte-Wells auf eine iBind Karte freigegeben. Die Glasfasermatrix der Karte ermöglicht einen homogenen und gleichmäßigen Fluss der Lösungen über die Membran und verstärkt damit die Antigen-Antikörper-Interaktion.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Fassungsvermögen1 Mini-Blot (8 x 8 cm)

Menge1 Kit

Laufzeit∼2,5 Stunden

NachweisverfahrenChromogen, chemolumineszierend, fluoreszierend

Membrane CompatibilityNitrozellulose, PVDF

ProduktlinieiBind

ProdukttypStarter-Kit

Unit Size1 kit

Inhalt und Lagerung

• 1 iBind™ Western-Gerät
• 10 iBind™ Karten (bei Raumtemperatur lagern)
• 1 iBind™ Lösungskit (bei 2 – 8 °C lagern)

Häufig gestellte Fragen (FAQ)

Do you offer additional years of service for the iBind Western Device?

No, we do not provide service contracts or extended warranties for these devices. The warranty is limited to 1 year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBind™ Western-Starterkit FAQs