Citations et références (3)

Gibco™

Pénicilline-streptomycine (10 000 U/ml)

Name: Pénicilline-streptomycine (10 000 U/ml)
SKU: 15140163
Price: 429.65 EUR

Cette solution contient 10 000 unités/ml de pénicilline et 10 000 μg/mL de streptomycine. Les antibiotiques pénicilline et streptomycine sontAfficher plus

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Référence	Quantité
15140163 également connu sous le numéro 15140-163	20 x 100 mL
15140148 également connu sous le numéro 15140-148	20 mL
15140122 également connu sous le numéro 15140-122	100 mL

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Référence 15140163

également connu sous le numéro 15140-163

Prix (EUR)

429,65

Online Exclusive

524,00

Économisez 94,35 (18%)

Each

Quantité:

20 x 100 mL

Prix (EUR)

429,65

Online Exclusive

524,00

Économisez 94,35 (18%)

Each

Cette solution contient 10 000 unités/ml de pénicilline et 10 000 μg/mL de streptomycine. Les antibiotiques pénicilline et streptomycine sont utilisés pour éviter la contamination bactérienne des cultures cellulaires en raison de leur action combinée effective contre les bactéries à Gram positif et à Gram négatif. À l’origine, la pénicilline a été purifiée à partir de champignons Penicillium et agit en interférant directement avec l’activité des parois des cellules bactériennes et indirectement en déclenchant la libération d’enzymes qui continuent à modifier la paroi cellulaire. À l’origine, la streptomycine a été purifiée à partir de Streptomyces griseus. Elle agit en se liant à la sous-unité 30S du ribosome bactérien, ce qui conduit à l’inhibition de la synthèse protéique et à la mort des bactéries sensibles.

Nous proposons une large gamme d’antibiotiques et d’antimycosiques aussi bien sous forme liquide qu’en poudre. En savoir plus sur les types de produits suivants :

• Antibiotiques de culture cellulaire
• Sélection des antibiotiques (y compris les concentrations de travail recommandées)

En savoir plus sur l’utilisation des antibiotiques et des antimycosiques dans la culture cellulaire et revoir les recommandations relatives à la décontamination des cultures.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Concentration100 X

À utiliser avec (application)Prévention de la contamination par culture cellulaire

Gamme de produitsGibco

Quantité20 x 100 mL

Durée de conservation12 mois

FormeLiquide

Type de produitAntibiotique

StérilitéStérilisation par filtration

Sterilization MethodStérilisation par filtration

Unit SizeEach

Contenu et stockage

Conditions de stockage : -5°C à -20°C
Conditions d’expédition : Glace carbonique
Durée de conservation : 12 mois à compter de la date de fabrication

Foire aux questions (FAQ)

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (3)

Citations et références

Abstract

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

Ferroptosis in Neurons and Cancer Cells Is Similar But Differentially Regulated by Histone Deacetylase Inhibitors.

Authors:Zille M, Kumar A, Kundu N, Bourassa MW, Wong VSC, Willis D, Karuppagounder SS, Ratan RR

Journal:

PubMed ID:30783618

Ferroptotic death is a mechanism for tumor suppression by pharmacological inhibitors that target the X

Phosphorylated STAT3 (Tyr705) as a biomarker of response to pimozide treatment in triple-negative breast cancer.

Authors:Dees S, Pontiggia L, Jasmin JF, Mercier I

Journal:Cancer Biol Ther

PubMed ID:32164483

Triple-negative breast cancer (TNBC) displays an aggressive clinical course, heightened metastatic potential, and is linked to poor survival rates. Through its lack of expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), this subtype remains unresponsive to traditional targeted therapies. Undesirable and ... More