EGS (bis(succinimidyl succinate) d’éthylène glycol)

Citations et références (5)

Thermo Scientific™

EGS (bis(succinimidyl succinate) d’éthylène glycol)

L’EGS Thermo Scientific Pierce est un réticulateur qui contient des extrémités avec esters de NHS réactives à l’amine autour d’unAfficher plus

Référence	Quantité
21565	1 g

Référence 21565

Prix (EUR)

279,00

Quantité:

Grand volume ou format personnalisé

Prix (EUR)

279,00

L’EGS Thermo Scientific Pierce est un réticulateur qui contient des extrémités avec esters de NHS réactives à l’amine autour d’un bras espaceur à 12 atomes, qui peut être clivé par traitement avec de l’hydroxylamine à un pH de 8,5.

Caractéristiques du réticulateur EGS :

• Groupes réactifs : Ester NHS (deux extrémités)
• Réactif vers : groupes amino (amines primaires)
• Hydrosoluble (dissoudre d’abord dans du DMF ou du DMSO) ; comparer au sulfo-EGS
• Perméable à la membrane, permettant une réticulation intracellulaire
• Les réticulations formées sont réversibles à un pH de 8,5 en utilisant de l’hydroxylamine pendant 3 à 6 heures à 37°C
• La lactose déshydrogénase a conservé 60 % de son activité après une réticulation réversible avec l’EGS

Références produit :
Guide d’application de la réticulation : recherche de références bibliographiques récentes pour ce produit

Produits associés
EGS (éthylène glycol bis(succinimidyl succinate))

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Perméabilité cellulaireOui

DescriptionEGS

FormePoudre

Méthode d’étiquetageMarquage chimique

Poids moléculaire456,36

PégyléNon

Gamme de produitsPierce

Quantité1 g

Groupement de réactifsEster NHS

Conditions d’expéditionTempérature ambiante

SolubilitéDMF, DMSO

Longueur du bras espaceur16,1 Å

HydrosolubleNon

Réactivité chimiqueAmine-Amine

CleavablePar hydroxylamine

Type d’agent de réticulationHomobifonctionnels

FormatÉtalon

Type de produitRéticulateur

EntretoiseMilieu (10 à 30 Å)

Unit SizeEach

Contenu et stockage

À la réception, conserver à 4°C.

Foire aux questions (FAQ)

Can you provide the shelf-life for EGS (ethylene glycol bis(succinimidyl succinate))?

EGS (ethylene glycol bis(succinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations et références (5)

Citations et références

Abstract

Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis.

Authors:Keinan N, Tyomkin D, Shoshan-Barmatz V

Journal:Mol Cell Biol

PubMed ID:20937774

'Accumulating evidence implicates that the voltage-dependent anion channel (VDAC) functions in mitochondrion-mediated apoptosis and as a critical player in the release of apoptogenic proteins, such as cytochrome c, triggering caspase activation and apoptosis. The mechanisms regulating cytochrome c release and the molecular architecture of the cytochrome c-conducting channel remain unknown. ... More

Structure-based analysis of VDAC1 protein: defining oligomer contact sites.

Authors:Geula S, Naveed H, Liang J, Shoshan-Barmatz V

Journal:J Biol Chem

PubMed ID:22117062

'The outer mitochondrial membrane protein, the voltage-dependent anion channel (VDAC), is increasingly implicated in the control of apoptosis. Oligomeric assembly of VDAC1 was shown to be coupled to apoptosis induction, with oligomerization increasing substantially upon apoptosis induction and inhibited by apoptosis blockers. In this study, structure- and computation-based selection of ... More

Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes.

Authors:Mokry M, Hatzis P, Schuijers J, Lansu N, Ruzius FP, Clevers H, Cuppen E

Journal:Nucleic Acids Res

PubMed ID:21914722

'Routine methods for assaying steady-state mRNA levels such as RNA-seq and micro-arrays are commonly used as readouts to study the role of transcription factors (TFs) in gene expression regulation. However, cellular RNA levels do not solely depend on activity of TFs and subsequent transcription by RNA polymerase II (Pol II), ... More

LXRa regulates macrophage arginase 1 through PU.1 and interferon regulatory factor 8.

Authors:Pourcet B, Feig JE, Vengrenyuk Y, Hobbs AJ, Kepka-Lenhart D, Garabedian MJ, Morris SM, Fisher EA, Pineda-Torra I

Journal:Circ Res

PubMed ID:21757649

Activation of liver X receptors (LXRs) inhibits the progression of atherosclerosis and promotes regression of existing lesions. In addition, LXRa levels are high in regressive plaques. Macrophage arginase 1 (Arg1) expression is inversely correlated with atherosclerosis progression and is markedly decreased in foam cells within the lesion. ... More

Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation.

Authors:Gogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D

Journal:J Biol Chem

PubMed ID:21712378

Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated translocation of Bax to mitochondria where it underwent oligomerization to initiate apoptosis. Resveratrol treatment promoted ... More