Sulfo-NHS (N-hydroxysulfosuccinimide), format No-Weigh™

Citations et références (5)

Thermo Scientific™

Sulfo-NHS (N-hydroxysulfosuccinimide), format No-Weigh™

Name: Sulfo-NHS (N-hydroxysulfosuccinimide), format No-Weigh™
SKU: 24510
Price: 440.00 EUR

Le Sulfo-NHS Thermo Scientific Pierce au format No-Weigh est un réactif de modification chimique pour convertir des groupes de carboxyleAfficher plus

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Référence	Quantité
24510	500 mg
A39269	10 x 2 mg
24525	5 g

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Référence 24510

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440,00

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Quantité:

500 mg

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440,00

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Le Sulfo-NHS Thermo Scientific Pierce au format No-Weigh est un réactif de modification chimique pour convertir des groupes de carboxyle en esters NHS réactifs aux amines pour les méthodes de bioconjugaison, réticulation, marquage et immobilisation.

Caractéristiques du Sulfo-NHS :

• L’efficacité du couplage induit par EDC est accrue en présence du Sulfo-NHS.
• Les esters NHS réactifs aux amines ou les esters Sulfo-NHS peuvent être fabriqués avec toute molécule à base de carboxyle
• En règle générale, les dérivés Sulfo-NHS sont directement hydrosolubles (ils peuvent être ajoutés directement aux tampons physiolo giques) et imperméables aux membranes (ils peuvent être utilisés pour le marquage de la surface cellulaire)
• Le Sulfo-NHS cristalline, d’une grande pureté, permet de créer des dérivés activés de qualité supérieure

Le Sulfo-NHS (N-hydroxysulfosuccinimide) permet le contrôle et la modification des réactions de réticulation carbodiimide qui supposent l’activation des carboxylates (—COOH) pour conjuguer les amines primaires (—NH2). Les dérivés sont facilement synthétisés en mélangeant le Sulfo-NHS à une molécule contenant le carboxyle et un agent déshydratant, comme l’EDC carbodiimide (EDAC). Cette méthode permet de générer plusieurs types de réactifs de marquage protéique, notamment les colorants fluorescents réactifs aux amines, les marquages d’affinité de la biotine et les composés de pégylation.

Applications :
• Améliorez l’efficacité des réactions de couplage EDC
• Convertissez les carboxyles en esters de sulfo-NHS réactifs aux amines
• Réticulez les protéines en microbilles ou surfaces revêtues de carboxyle plus efficacement
• Activez des nanoparticules avec des esters de sulfo-NHS réactifs aux amines

Spécifications pour le Sulfo-NHS :
nous fabriquons du N-hydroxysulfosuccinimide selon les spécifications les plus élevées possibles pour produire les bioconjugués les plus spécifiques, pour assurer l’intégrité de vos données et pour vous fournir le plus haut degré de cohérence. Chaque lot de Sulfo-NHS est testé pour satisfaire les spécifications minimales suivantes :

• Pureté : 95 % supérieure par résonance magnétique nucléaire (RMN) quantitative (norme optimale pour assurer la pureté de réticulation) ;
la pureté moyenne du lot est 99 % supérieure
• Solubilité : l’échantillon se dissout à 2 mg/ml dans l’eau déionisée pour obtenir une solution transparente et incolore
• Identité : le balayage IR montre uniquement des pics caractéristiques de N-hydroxysulfosuccinimide

Références du produit :
Guide d’application des agents de réticulation -- recherche de références bibliographiques récentes pour ce produit

Produits associés
Sulfo-NHS (N-hydroxysulfosuccinimide) de qualité supérieure Pierce™
NHS (N-hydroxysuccinimide)
EDC (1-éthyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Perméabilité cellulaireNon

DescriptionSulfo-NHS

FormePoudre

Méthode d’étiquetageMarquage chimique

Poids moléculaire217,13

PégyléNon

Gamme de produitsPierce

Quantité500 mg

Groupement de réactifsCarbodiimide

Conditions d’expéditionTempérature ambiante

SolubilitéEau

HydrosolubleOui

Réactivité chimiqueAmine-Carboxyle

CleavableNon

Type d’agent de réticulationHétérobifonctionnel

FormatStandard, usage unique, qualité supérieure

Type de produitRéticulateur

EntretoiseCourt (< 10 Å)

Unit SizeEach

Contenu et stockage

À la réception, conserver à 4°C.

Foire aux questions (FAQ)

Can NHS and EDC stock solutions be made for long-term storage before use?

No. EDC is very unstable in aqueous environments and must be dissolved immediately before use. NHS and Sulfo-NHS are relatively stable in solution but best results are obtained when they are dissolved immediately before use. Store these compounds desiccated at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are there any substances that interfere with NHS reactions?

Yes. Except for the intended targets, reactions must not contain carboxyl or amine compounds. Thus, Tris, glycine, lysine, ethanolamine or other amine- containing buffers must be avoided.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between NHS and Sulfo-NHS?

Sulfo-NHS is the sulfonate sodium salt of NHS; it is water-soluble, but not membrane-permeable. NHS is membrane-permeable and water-soluble. NHS is soluble in organic solvents, as is Sulfo-NHS to a lesser extent. Because NHS is a leaving group in reactions with primary amines, the final conjugation product resulting from NHS and Sulfo-NHS reactions is identical. Sulfo-NHS is chosen for its ability to confer better solubility to the activated compound and/or to control its membrane permeability.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How are NHS and Sulfo-NHS used?

These compounds are used to modify a carboxyl group to form an amine-reactive ester. This is accomplished by mixing NHS with a carboxyl-containing molecule and the carbodiimide EDC (Cat. No. 22980, 22981, 77149, A35391). EDC causes a dehydration reaction between the carboxyl and the NHS hydroxyl group, giving rise to an NHS-ester-activated molecule. The activated molecule can then be reacted spontaneously with a primary amine-containing molecule. Although the carboxyl-molecules can be made to react directly with amines using EDC, the reaction is much more efficient with NHS because a stable intermediate is created. In fact, molecules that are activated as NHS esters can be dried and stored for later reaction to amine-containing targets.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are NHS and Sulfo-NHS used for?

These compounds are used in conjunction with the crosslinker EDC (Cat. No. 22980, 22981, 77149, A35391) to synthesize amine-reactive labeling reagents, crosslinkers and conjugation compounds. Any compound containing a carboxylic acid (-COOH), such as a protein, or biotin or peptide, can be activated with NHS or Sulfo-NHS to form an NHS ester that will spontaneously react to form covalent amide linkages with proteins and other molecules that contain primary amines (-NH2).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Sulfo-NHS (N-hydroxysulfosuccinimide), format No-Weigh™ FAQs

Citations et références (5)

Citations et références

Abstract

Investigation of platelet responses and clotting characteristics of in situ albumin binding surfaces.

Authors:Guha Thakurta S, Miller R, Subramanian A

Journal:J Biomater Appl

PubMed ID:20819918

'The response of biomaterial surfaces when exposed to blood is in part dependent upon the nature and composition of the adsorbed layer of proteins. Surfaces passivated with albumin have been shown to reduce platelet adhesion and activation. In an attempt to develop surfaces that can selectively and specifically bind albumin, ... More

Mouse monoclonal antibodies to anthrax edema factor protect against infection.

Authors:Leysath CE, Chen KH, Moayeri M, Crown D, Fattah R, Chen Z, Das SR, Purcell RH, Leppla SH

Journal:Infect Immun

PubMed ID:21911463

'Bacillus anthracis is the causative agent of anthrax, and the tripartite anthrax toxin is an essential element of its pathogenesis. Edema factor (EF), a potent adenylyl cyclase, is one of the toxin components. In this work, anti-EF monoclonal antibodies (MAb) were produced following immunization of mice, and four of the ... More

Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid.

Authors:Bergfeld AK, Pearce OM, Diaz SL, Pham T, Varki A

Journal:J Biol Chem

PubMed ID:22692205

'The two major mammalian sialic acids are N-acetylneuraminic acid and N-glycolylneuraminic acid (Neu5Gc). The only known biosynthetic pathway generating Neu5Gc is the conversion of CMP-N-acetylneuraminic acid into CMP-Neu5Gc, which is catalyzed by the CMP-Neu5Ac hydroxylase enzyme. Given the irreversible nature of this reaction, there must be pathways for elimination or ... More

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles.

Authors:Sperling RA, Parak WJ

Journal:Philos Trans A Math Phys Eng Sci

PubMed ID:20156828

Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the ... More

The conserved His-144 in the PsbP protein is important for the interaction between the PsbP N-terminus and the Cyt b559 subunit of photosystem II.

Authors:Ido K, Kakiuchi S, Uno C, Nishimura T, Fukao Y, Noguchi T, Sato F, Ifuku K

Journal:J Biol Chem

PubMed ID:22707728

The PsbP protein regulates the binding properties of Ca(2+) and Cl(-), and stabilizes the Mn cluster of photosystem II (PSII); however, the binding site and topology in PSII have yet to be clarified. Here we report that the structure around His-144 and Asp-165 in PsbP, which is suggested to be ... More