DMEM, high glucose
DMEM, high glucose
Citations & References (14)
Gibco™

DMEM, high glucose

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
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Catalog NumberQuantity
119651266 x 1000 mL
11965092500 mL
1196511810 x 500 mL
119650841000 mL
119651675 L
1196517510 L
Catalog number 11965126
Price (JPY)
24,700
Each
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Quantity:
6 x 1000 mL
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
For Use With (Application)Mammalian Cell Culture
Glucose Concentration4500 mg/L
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity6 x 1000 mL
Shelf Life12 Months From Date of Manufacture
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

What is the osmolality of DMEM, high glucose (Cat. No. 11965xxx)?

We do provide osmolality information on the certificate of analysis. All lots of DMEM, high glucose (Cat. No. 11965xxx) will meet the osmolality specification of 320-355 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, high glucose FAQs

Citations & References (14)

Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Efficacy of acetaminophen in skin B16-F0 melanoma tumor-bearing C57BL/6 mice.
Authors:Vad NM, Kudugunti SK, Graber D, Bailey N, Srivenugopal K, Moridani MY
Journal:Int J Oncol
PubMed ID:19513568
'Previously, we reported that acetaminophen (APAP) showed selective toxicity towards melanoma cell lines. In the current study, we investigated further the role of tyrosinase in APAP toxicity in SK-MEL-28 melanoma cells in the presence of a short hairpin RNA (shRNA) plasmid, silencing tyrosinase gene. Results from tyrosinase shRNA experiments showed ... More
Calcium regulation of matrix metalloproteinase-mediated migration in oral squamous cell carcinoma cells.
Authors:Munshi HG, Wu YI, Ariztia EV, Stack MS,
Journal:J Biol Chem
PubMed ID:12194986
'Activation of matrix metalloproteinase 2 (MMP-2) has been shown to play a significant role in the behavior of cancer cells, affecting both migration and invasion. The activation process requires multimolecular complex formation involving pro-MMP-2, membrane type 1-MMP (MT1-MMP), and tissue inhibitor of metalloproteinases-2 (TIMP-2). Because calcium is an important regulator ... More
Characterization of the human forkhead gene FREAC-4. Evidence for regulation by Wilms' tumor suppressor gene (WT-1) and p53.
Authors: Ernstsson S; Pierrou S; Hulander M; Cederberg A; Hellqvist M; Carlsson P; Enerbäck S;
Journal:J Biol Chem
PubMed ID:8702877
'We describe the cloning and sequence analysis of a nearly full-length cDNA as well as a corresponding 5.2-kilobase pair genomic fragment encoding FREAC-4, a member of the forkhead family of transcription factors. The cDNA is collinear with respect to the coding region of the intronless genomic clone. The conceptual translation ... More
Foam cell formation inhibits growth of Chlamydia pneumoniae but does not attenuate Chlamydia pneumoniae-induced secretion of proinflammatory cytokines.
Authors: Blessing Erwin; Kuo Cho-Chou; Lin Tsun-Mei; Campbell Lee Ann; Bea Florian; Chesebro Brian; Rosenfeld Michael E;
Journal:Circulation
PubMed ID:11997286
'BACKGROUND: It has not yet been determined whether lipid-loaded macrophages (foam cells), a major cellular component of atherosclerotic lesions, have the capacity to support growth of Chlamydia pneumoniae and be activated to secrete proinflammatory cytokines in response to C pneumoniae infection. METHODS AND RESULTS: Lipid loading of RAW 264.7 cells ... More
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