HEPES (1M)

Citations & References (5)

Gibco™

HEPES (1M)

Name: HEPES (1M)
SKU: 15630106

Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It is membrane impermeable, relatively stable, and has limited interference in key processes.

Technical SupportCustomer Service

Change view

Catalog Number	Quantity
15630106	20 mL
15630080	100 mL
15630130	20 x 100 mL

3 Options

Catalog number 15630106

Price (JPY)

8,400

Each

Quantity:

20 mL

Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO₂ incubator.

Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a good buffering choice for many cell culture systems because it:

Is membrane impermeable
Has limited effect on biochemical reactions
Is chemically and enzymatically stable
Has a very low visible and UV light absorbance

cGMP manufacturing and quality system

For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY, and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Chemical Name or MaterialZwitterionic Organic Chemical Buffer

FormulationN-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid

Recommended StorageStorage conditions: 2 – 8°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

SterilitySterile

Sterilization MethodSterile-filtered

For Use With (Application)Chromatin Biology

GradeBiochemical

Physical FormLiquid

Quantity20 mL

Solution TypeBuffer

Unit SizeEach

Frequently asked questions (FAQs)

Based on the Sodium bicarbonate levels in the medium, what CO2 % is recommended?

If the media formulation contains:

NaHCO3 (g/L) < 1.5, it needs CO2 at 4%;
NaHCO3 (g/L) 1.5 – 2.2, it needs CO2 at 5%;
NaHCO3 (g/L) 2.2 - 3.4, it needs CO2 at 7%;
NaHCO3 (g/L) > 3.5, it needs CO2 at 10% .

*There are some exceptions. Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7 g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. This also depends on the cell type. Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do high or low levels of sodium bicarbonate affect the pH of my tissue culture media?

Sodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator*.

If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH.

If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.

*There are some exceptions. For instance, Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. this also depends on the cell type, Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My Gibco HEPES (1M) (Cat. No. 15630080) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is HEPES (1 M) in distilled water or in saline solution?

1M HEPES is in water with a pH range of 7.2 - 7.5.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the endotoxin specification for HEPES (1 M) (Cat. Nos. 15630080, 15630106, and 15630130)?

Sorry, we do not test endotoxin levels for this product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (5)

Citations & References

Abstract

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes.

Authors:Alawar N,Schirra C,Hohmann M,Becherer U

Journal:BMC biotechnology

PubMed ID:38532411

BACKGROUND: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized, electroporation remains the preferred technique for transient gene over-expression. However, the efficiency of electroporation is reduced for human and ... More

Thiazolidinedione activation of peroxisome proliferator-activated receptor gamma can enhance mitochondrial potential and promote cell survival.

Authors: Wang Y Lynn; Frauwirth Kenneth A; Rangwala Shamina M; Lazar Mitchell A; Thompson Craig B;

Journal:J Biol Chem

PubMed ID:12082115

'Thiazolidinediones (TZDs) are widely used for treatment of type 2 diabetes mellitus. Peroxisome proliferator-activated receptor gamma (PPAR gamma) is the molecular target of TZDs and is believed to mediate the apoptotic effects of this class of drugs in a variety of cell types, including B and T lymphocytes. The finding ... More

Role of hepatic transporters in the disposition and hepatotoxicity of a HER2 tyrosine kinase inhibitor CP-724,714.

Authors:Feng B, Xu JJ, Bi YA, Mireles R, Davidson R, Duignan DB, Campbell S, Kostrubsky VE, Dunn MC, Smith AR, Wang HF,

Journal:Toxicol Sci

PubMed ID:19223659

'CP-724,714, a potent and selective orally active HER2 tyrosine kinase inhibitor, was discontinued from clinical development due to unexpected hepatotoxicity in cancer patients. Based on the clinical manifestation of the toxicity, CP-724,714 likely exerted its hepatotoxicity via both hepatocellular injury and hepatobiliary cholestatic mechanisms. The direct cytotoxic effect, hepatobiliary disposition ... More

Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.

Authors: Sobolevsky Alexander I; Beck Christine; Wollmuth Lonnie P;

Journal:Neuron

PubMed ID:11779481

Many N-methyl-D-aspartate receptor (NMDAR) channel blockers that have therapeutic potential can be trapped in the closed state. Using a combination of the substituted cysteine accessibility method and open channel blockers, we found that the M3 segment forms the core of the extracellular vestibule, including a deep site for trapping blockers. ... More

Activation of human monoamine oxidase B gene expression by a protein kinase C MAPK signal transduction pathway involves c-Jun and Egr-1.

Authors: Wong Wai K; Ou Xiao-Ming; Chen Kevin; Shih Jean C;

Journal:J Biol Chem

PubMed ID:11956220

Monoamine oxidases (MAO) A and B deaminate a number of biogenic amines. Aberrant expression of MAO is implicated in several psychiatric and neurogenerative disorders. In this study, we have shown that phorbol 12-myristate 13-acetate (PMA) increases human MAO B, but not MAO A, gene expression. The sequence between -246 and ... More