McCoy's 5A (Modified) Medium
McCoy's 5A (Modified) Medium
Gibco™

McCoy's 5A (Modified) Medium

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, establishedRead more
Catalog NumberQuantity
16600082500 mL
1660010810 x 500 mL
2 Options
Catalog number 16600082
Price (JPY)/ 500 mL
Price:2,900
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500 mL
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McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.


This McCoy's 5A is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Bacto-peptone 
• Phenol Red 


The complete formulation is available.

Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications
Cell LineRat fibroblasts
Cell TypeBiopsy Tissues
ClassificationAnimal Origin
Concentration1 X
FormLiquid
Product TypeMcCoy's 5A Modified Medium
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
Without AdditivesNo HEPES, No Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit Size500 mL
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture