Opti-MEM™ Reduced Serum Medium, powder

Citations & References (6)

Gibco™

Opti-MEM™ Reduced Serum Medium, powder

Name: Opti-MEM™ Reduced Serum Medium, powder
SKU: 22600050

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementationRead more

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Catalog Number	Quantity
22600050	10 x 1 L
22600134	10 L

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Catalog number 22600050

Price (JPY)

46,600

Each

Quantity:

10 x 1 L

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Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™ modifications for a range of cell culture applications.

This Opti-MEM™ is modified as follows:

With

• L-glutamine

• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.

Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.

Specifications

Cell LineSp2, AE-1, CHO, BHK-21, and HEK

Cell TypePrimary Fibroblasts

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Product LineGibco, Opti-MEM

Product TypeOpti-MEM I

Quantity10 x 1 L

Shelf Life30 Months From Date of Manufacture

Shipping ConditionRoom Temperature

ClassificationAnimal Origin

FormPowder

SterilitySterile-filtered

With AdditivesGlutamine, Phenol Red

Unit SizeEach

Contents & Storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 30 months from date of manufacture

Frequently asked questions (FAQs)

Do you offer an alternative for Opti-MEM Reduced Serum Medium powder (Cat. No. 22600050)?

The direct alternative for Opti-MEM Reduced Serum Medium powder (Cat. No. 22600050) is Opti-MEM Reduced Serum Medium powder (Cat. No. 22600134). The product is the same but comes in a 10 L volume. Therefore, you might consider reconstituting the powder and then aliquoting the resulting media into smaller bottles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Opti-MEM Reduced Serum Medium, powder (Cat. No. 22600050, 22600134) should I use to reconstitute the media?

We recommend reconstituting Opti-MEM Reduced Serum Medium, powder (Cat. No. 22600050, 22600134) at 13.592 g/L.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ Reduced Serum Medium, powder, 10 x 1 L FAQs

Citations & References (6)

Citations & References

Abstract

Folding of the striated muscle myosin motor domain.

Authors: Chow Diana; Srikakulam Rajani; Chen Ying; Winkelmann Donald A;

Journal:J Biol Chem

PubMed ID:12110670

'We have investigated the folding of the myosin motor domain using a chimera of an embryonic striated muscle myosin II motor domain fused on its COOH terminus to a thermal stable, fast folding variant of green fluorescent protein (GFP). In in vitro expression assays, the GFP domain of the chimeric ... More

Role of the E1A Rb-binding domain in repression of the NF-kappa B-dependent defense against tumor necrosis factor-alpha.

Authors: Cook James L; Walker Thomas A; Worthen G Scott; Radke Jay R;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12119420

'The adenoviral E1A oncogene sensitizes mammalian cells to tumor necrosis factor-alpha (TNF-alpha), in part by repressing the nuclear factor-kappa B (NF-kappa B)-dependent defense against this cytokine. Other E1A activities involve binding to either p300/cyclic AMP response element-binding protein (CBP) or retinoblastoma (Rb)-family proteins, but the roles of E1A interactions with ... More

Induction of bacterial lipoprotein tolerance is associated with suppression of toll-like receptor 2 expression.

Authors:Wang JH, Doyle M, Manning BJ, Di Wu Q, Blankson S, Redmond HP.

Journal:J Biol Chem

PubMed ID:12133836

'Tolerance to bacterial cell wall components including lipopolysaccharide (LPS) may represent an essential regulatory mechanism during bacterial infection. Two members of the Toll-like receptor (TLR) family, TLR2 and TLR4, recognize the specific pattern of bacterial cell wall components. TLR4 has been found to be responsible for LPS tolerance. However, the ... More

Hepatitis C virus subgenomic replicons in the human embryonic kidney 293 cell line.

Authors:Ali S, Pellerin C, Lamarre D, Kukolj G,

Journal:J Virol

PubMed ID:14671129

'Hepatitis C virus (HCV) infects liver cells and its replication in other cells is incompletely defined. Human hepatoma Huh-7 cells harboring subgenomic HCV replicons were used in somatic cell fusion experiments with human embryonic kidney 293 cells as a means of examining the permissiveness of 293 cells for HCV subgenomic ... More

c-Myc sensitizes cells to tumor necrosis factor-mediated apoptosis by inhibiting nuclear factor kappa B transactivation.

Authors: You Zongbing; Madrid Lee V; Saims Daniel; Sedivy John; Wang Cun-Yu;

Journal:J Biol Chem

PubMed ID:12149248

Nuclear factor kappaB (NF-kappaB) plays a key role in suppression of tumor necrosis factor (TNF)-mediated apoptosis by inducing a variety of anti-apoptotic genes. Expression of c-Myc has been shown to sensitize cells to TNF-mediated apoptosis by inhibiting NF-kappaB activation. However, the precise step in the NF-kappaB signaling pathway and apoptosis ... More

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