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Pierce™ Peroxide Assay Reagent B
Thermo Scientific Pierce Peroxide Assay Reagent B is a formulation consisting of 125μM xylenol orange in water with sorbitol. ThisRead more
| Catalog Number | Quantity |
|---|---|
| 23282 | 50 mL |
Catalog number 23282
Price (JPY)Contact Us ›
34,300
Each
Quantity:
50 mL
Thermo Scientific Pierce Peroxide Assay Reagent B is a formulation consisting of 125μM xylenol orange in water with sorbitol. This formulation is one of the component of Quantitative Peroxide Assay Kit used for detecting and measuring hydrogen peroxide levels based on the oxidation of ferrous to ferric ion in the presence of xylenol orange, present in the biological and other liquid samples.Convenient—no proprietary reagents involved and remove the variability, difficulty and added expense of formulating the reagents yourself Aqueous-compatible formulations— the formulation includes sorbitol, which provides enhanced sensitivity with non-lipid samples General hydrogen peroxide (H2O2) measurement in protein samples Quantitation of peroxides in detergents Assessment of the effects of peroxides on cellular activity Determination of protein glycation; Measurement of peroxide accumulation in lipids
Features of Peroxide Assay Reagent B:
Applications of Pierce Quantitative Peroxide Assay Kits:
In these assay kits, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.
Related Products
Pierce™ Quantitative Peroxide Assay Kit (Aqueous)
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayPeroxide Assay Reagent B
DescriptionPierce Peroxide Assay Reagent B
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Product LinePierce
Product TypeQuantitative Peroxide Assay
Quantity50 mL
SpecificityNot Target-Specific
Detection MethodColorimetric
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.