BigDye™ Terminator Plus Cycle Sequencing Kit

The BigDye™ Terminator BDT Plus Cycle Sequencing Kit is a Sanger sequencing master mix designed to enable faster results and improved performance across a broad range of sample types. Built on a newly developed high-performance enzyme, BDT Plus enables more efficient sequencing of challenging templates while maintaining full compatibility with existing BigDye™ workflows and capillary electrophoresis (CE) instruments. Compared to legacy BigDye™ Terminator v3.1 chemistry, BDT Plus helps provide enhanced speed, flexibility, and reliability without requiring workflow changes.

Key Features and Benefits

• Accelerate sequencing workflows with up to ~50% faster time to results
• Improve performance on challenging templates using an advanced high-processivity enzyme
• Maintain existing workflows with full compatibility across current BigDye™ protocols and CE instruments
• Increase success rates and reduce re-runs for greater laboratory efficiency
• Flexible Protocol Optimization: Supports implementation of customer-optimized workflows, allowing laboratories to maintain validated protocols and specialized applications

Product Details

The BDT Plus kit incorporates a newly engineered enzyme with increased processivity, allowing for more efficient incorporation of dye-labeled terminators during cycle sequencing. This enhanced enzyme performance improves read quality and consistency, particularly when working with difficult templates such as GC-rich regions, secondary structures, or low-quality samples. The optimized formulation supports robust sequencing across a wider range of sample inputs and conditions.

BDT Plus is designed as a drop-in replacement for existing BigDye™ Terminator chemistries, requiring no changes to established workflows. Researchers can integrate the kit directly into their current cycle sequencing protocols, using the same instruments, plastics, and cleanup methods. This compatibility reduces disruption while enabling immediate performance improvements.

The kit supports a wide range of Sanger sequencing research applications, including assay development, mutation detection, plasmid verification, and confirmatory sequencing. It is well suited for use in clinical research, biopharma, academic, and core laboratory environments, where sample diversity and throughput requirements vary. Its ability to process challenging and heterogeneous sample types makes it particularly valuable for labs seeking consistent results across complex workflows.

By combining faster turnaround times with improved sequencing performance, BDT Plus helps laboratories increase productivity, reduce repeat runs, and maintain confidence in their sequencing data.