GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Catalog Number	Quantity
4379876	2 x 75 mL

Catalog number 4379876

Price (JPY)

32,700

Quantity:

2 x 75 mL

Request bulk or custom format

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification. GeneAmp 10X PCR Buffer I contains 15 mM MgCl₂. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq DNA Polymerase.

GeneAmp 10X PCR Buffer I is available in several convenient sizes and when used with AmpliTaq DNA Polymerase offers maximum PCR performance.

Note

See user's manual or package insert for limited label license and trademark information.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq)1X

PolymeraseAmpliTaq DNA Polymerase

Product TypePCR Buffer

Quantity2 x 75 mL

Reaction FormatKit

Shipping ConditionWet Ice

Volume150 mL

Concentration10X

For Use With (Application)Standard PCR

Unit SizeEach

Contents & Storage

• GeneAmp 10X PCR Buffer I (with 15 mM MgCl₂), 2 x 75 mL

Store at -15°C to -25°C.

Frequently asked questions (FAQs)

How many freeze cycles do you recommend for Applied Biosystems, GeneAmp 10X PCR Buffer I (Cat. No. 4379876)?

We do not have specific guidance on the number of freeze thaws. We recommend following the guidance for aliquoting 1.5 mL of this buffer into autoclaved 2 mL tubes after the first thaw as described in Product Insert: GeneAmp 10X PCR Buffer I. This will help increase efficiency when working with the product and serve to limit the number of freeze-thaws for the buffer.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What are the differences between GeneAmp 10X PCR Buffer I, PCR Buffer II and PCR Gold Buffer, and what are the formulations of each buffer?

The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been carefully optimized for use with the AmpliTaq Gold enzyme. GeneAmp 10X PCR Buffer I contains Mg; Geneamp 10X PCR Buffer II and Geneamp 10X PCR Gold Buffer do not have Mg in them, but come with a separate vial of 25 mM MgCl2 so that you can titrate the Mg in your PCR reaction.

Buffer formulations:
Buffer I (500 mM KCl, 100 mM Tris-HCl, pH 8.3, 15 mM MgCl2, 0.01% (w/v) gelatin)
Buffer II (500 mM KCl, 100 mM Tris-HCl, pH 8.3), with separate tube of 25 mM MgCl2
Gold Buffer (500 mM KCl, 150 mM Tris-HCl pH 8.0), with separate tube of 25 mM MgCl2

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.