AmpliTaq Gold™ 360 Buffer Kit

The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient.

Catalog Number	Quantity
4398853	1 Kit

Catalog number 4398853

Price (JPY)

12,100

Quantity:

1 Kit

Request bulk or custom format

The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for use on occasions when the quantity of reagents supplied with the enzyme are not sufficient.

This kit includes 1.5 mL (each) of:

10X AmpliTaq Gold 360 Buffer
25 mM MgCl₂
360 GC Enhancer

Typically, 1.5 mL is sufficient for use with 250 U of enzyme.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq)1X

FormatKit

PolymeraseAmpliTaq Gold 360 DNA Polymerase

Product TypePCR Buffer

Quantity1 Kit

Shipping ConditionRoom Temp or Wet Ice

Volume1.5 mL

Concentration10X

For Use With (Application)Cloning

Unit SizeEach

Contents & Storage

• 10X AmpliTaq Gold 360 Buffer, 1 x 1.5 mL
• 25 mM MgCl₂, 1 x 1.5 mL
• 360 GC Enhancer, 1 x 1.5 mL

Store at -15° to -25°C.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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