Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR)

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour.

Catalog Number	Quantity
4403087	1 kit

Catalog number 4403087

Price (JPY)

859,600

Quantity:

1 kit

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour. It combines DNA extraction and TaqMan chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

Provides PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
The TaqMan GTXpress Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
Compatible with both Fast and Standard mode thermal cycling conditions
Pre-mixed components stored at 2–8°C significantly reduce assay setup time
Validated with all types of TaqMan Genotyping assays
Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 μL/reaction and 20,000 PCR reactions at 5 μL for each reaction.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode

Genotyping TargetSNPs (Unknown or Numerous), SNPs (Known)

Hot StartBuilt-In Hot Start

No. of Reactions4000 Reactions (5 μL, PCR), 4000 Reactions (50 μL/Reaction, Sample Preparation)

Passive Reference DyeROX (Pre-mixed)

PolymeraseAmpliTaq DNA Polymerase

Product LineSample-to-SNP, TaqMan

Product TypePCR Kit

Quantity1 kit

Sample TypeBuccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood

TechniqueSNP Genotyping (Real-Time PCR-Based)

Volume20 mL (Sample Preparation), 50 mL (PCR)

Detection MethodPrimer-probe

PCR MethodqPCR

Reaction SpeedFast or Standard

Unit SizeEach

Contents & Storage

Contains Lysis Reagents (2 -8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25°C): 50 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Frequently asked questions (FAQs)

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".