The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.
• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min • The TaqMan™™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays • Extended PCR cycling for poorly amplified or sample-limited reactions
The kit includes reagents sufficient for 100 sample preparation reactions at 50 μL/ reaction and 400 PCR reactions at 5 μL for each reaction.
Please note: The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan™ Sample-to-SNP™ Kit.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
GC-Rich PCR Performance
Low
PCR Method
qPCR
Polymerase
AmpliTaq DNA Polymerase
Reaction Speed
Fast or Standard
Technique
SNP Genotyping (Real-Time PCR-Based)
For Use With (Equipment)
7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode
Lysis Reagents (2° to 8°C): 5 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C ): 1 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).