PBS (10X), pH 7.4

Citations & References (2)

Gibco™

PBS (10X), pH 7.4

Name: PBS (10X), pH 7.4
SKU: 70011069

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such asRead more

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Catalog Number	Quantity
70011069	10 x 500 mL
70011044	500 mL

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Catalog number 70011069

Price (JPY)

47,500

Each

Quantity:

10 x 500 mL

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details).

We offer a variety of Gibco™ PBS formulations for a range of cell culture applications.

This PBS is manufactured as follows:

Without

• Calcium

• Magnesium

• Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Chemical Name or MaterialPhosphate Buffered Saline (PBS)

ColorWhite

IncludesNo Sodium Pyruvate

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality2800 - 3100 mOsm/kg

Recommended StorageStorage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

SterilitySterile-filtered

Concentration10 X

For Use With (Application)Mammalian Cell Culture

Physical FormLiquid

Product LineGibco

Quantity10 x 500 mL

Solution TypePhosphate Buffered Saline

pH7.4

Unit SizeEach

Frequently asked questions (FAQs)

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is a precipitate in PBS normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between PBS and DPBS?

PBS has no potassium chloride (KCl) and it has a slightly lower level of phosphate (PO4) than DPBS. PBS is used for washing cells when viability isn't critical.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (2)

Citations & References

Abstract

Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior.

Authors:Fischer RS, Myers KA, Gardel ML, Waterman CM,

Journal:Nat Protoc

PubMed ID:23099487

Regulation of cell functions by the physical properties of the extracellular matrix (ECM) has emerged as a crucial contributor to development and disease. Two specific physical properties of the ECM, stiffness and dimensionality, each influence cell signaling and function. As these ECM physical properties are linked to other properties that ... More

Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.

Authors:Zhang F, Gradinaru V, Adamantidis AR, Durand R, Airan RD, de Lecea L, Deisseroth K,

Journal:Nat Protoc

PubMed ID:20203662

Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity ... More