Invitrogen Collibri Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries. The master mix includes Platinum SuperFi DNA Polymerase in combination with a proprietary reaction buffer containing all necessary components for efficient library amplification with minimal bias and superior accuracy.

Feature include:
• High fidelity—minimal error rates similar to PCR-free methods
• High efficiency—high yields from minute input amounts
• Minimal bias—uniform amplification regardless of GC content

This Collibri Library Amplification Master Mix is supplied with a 10X Primer Mix that targets the P5 and P7 regions of Illumina adapters. The master mix is supplemented with an inert blue dye, while the primer mix contains a yellow dye. Mixing both components in a PCR reaction turns the final PCR solution green. This provides a visual aid when pipetting and helps decrease the risk of pipetting errors during reaction setup.

Platinum SuperFi DNA Polymerase has both 5’ to 3’ polymerase and 3’ to 5’ exonuclease (proofreading) activities, but lacks 5’ to 3’ exonuclease activity. Exceptionally strong proofreading activity ensures amplification of NGS libraries with supreme sequence accuracy, producing NGS error rates similar to PCR-free methods.

The proprietary reaction buffer contains all necessary PCR components and additives to enable efficient and uniform library amplification regardless of GC content, helping improve coverage across GC- and AT-rich sequences and other complex regions. High amplification efficiency enables the use of fewer cycles to achieve the desired yield.

Platinum hot-start technology inhibits DNA polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of pre-assembled PCR reactions for up to 24 hours prior to the PCR.