Sequential DNase I, 100 rxns

Applied Biosystems Sequential DNase I is a component of the Sequential Protein/DNA/RNA Extraction Kit used for DNA digestion in the RNA purification step.

Features of the Sequential Protein/DNA/RNA Extraction Kit include:

• High sensitivity—supports scalable extractions from precious or low sample volumes down to 1,000 cells or 0.005 mg tissue (tissue dependent)
• Direct compatibility—extracted analytes down to 100,000 cells or 1-2 mg tissue are validated and suitable for mass spectrometry and NGS applications
• Automated high-throughput—compatible with KingFisher instruments for scalable processing
• Enhanced reproducibility—standardized protocol reduces variability

The kit employs a bead-based sequential extraction methodology utilizing protein isolation prior to nucleic acid extraction, resulting in enhanced protein yields that are directly compatible with mass spectrometry analysis. This approach enables researchers to obtain analytes suitable for diverse downstream applications including SDS-PAGE, western blotting, mass spectrometry, sequencing, genotyping, and qPCR from sample inputs as small as 1-2 mg of tissue or 100,000 cells while maintaining quality standards required for both next-generation sequencing (NGS) and MS platforms. The Dynabeads and MagMAX magnetic bead-based purification technology helps enable high reproducibility and scalable, high-throughput processing when integrated with automation systems such as the KingFisher Sample Purification System.

This kit combines the advantages of multi-analyte extraction with the precision and efficiency required for advanced proteomics and genomics workflows, making it particularly valuable for multi-omics studies where sample conservation and analytical comprehensiveness are paramount.

Verified performance

The kit helps deliver yield and quality metrics comparable to standard single-analyte extraction kits, facilitating efficient multi-analyte recovery from a single sample. While most commercial sequential protocols are optimized for large sample volumes (e.g. 1 million cells or 10–20 mg tissue), this kit offers exceptional versatility across a wider range. Validated for 100,000 cells and 1–2 mg tissue, it has also been successfully used with as few as 1,000 cells and 5 μg tissue while maintaining linear yields and preserving nucleic acid integrity suitable for qPCR analysis. This broad dynamic range provides greater flexibility and enables efficient processing of precious or limited samples that are difficult to analyze with conventional methods.

Streamlined three-step sequential extraction

The extraction procedure follows a systematic three-step process designed to support a high recovery of each biomolecule class. Initially, Dynabeads Protein Beads are introduced to the lysed sample and incubated to facilitate protein binding, after which they are transferred to a separate tube for washing and protein processing compatible with downstream western blot or mass-spectrometry applications. Subsequently, MagMAX DNA/RNA Binding beads are added to the original tube, now depleted of proteins, to enable DNA binding and extraction using proprietary buffer formulations before transferring to a new tube for further preparation. Finally, MagMAX DNA/RNA Binding beads are again introduced to the original tube, now depleted of both proteins and DNA, to facilitate RNA binding and extraction using specialized proprietary buffers and solutions, with subsequent transfer to a dedicated tube for final preparation. The resulting extracted proteins are suitable for WB or MS analysis, while the recovered DNA and RNA are compatible with quantitative PCR (qPCR) and next-generation sequencing (NGS) applications.