TaqMan™ MGB Probe

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/Minor Groove Binder (MGB). Choose from FAM, VIC, ABY, JUN, Cyanine 5, TET, and NED as reporters. Probes are HPLC-purified.

Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5' fluorescent reporter dye and a 3' nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. Custom TaqMan MGB probes are HPLC-purified and available with FAM, VIC, ABY, JUN, Cyanine 5, TET, and NED reporter dyes.

Shorter, more specific probes

TaqMan MGB probes include a MGB moiety at the 3' end that increases the melting temperature (Tm) of the probe and stabilizes probe/target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.

Nonfluorescent quencher (NFQ) maximizes sensitivity

TaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.

Multiplexing

The availability of MGB probes for up to five channels allows for higher-order multiplexing, enabling detection of more targets from the same samples.

Guaranteed yield

The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.

Approximate ship time

4–10 days in North America, 5-10 days in Europe

We also offer:

• Applied Biosystems custom primers and other types of TaqMan probes.
• TaqMan MGB probes and primers available as predesigned and pre-formulated TaqMan Assays.
• Commercial manufacturing products and services, including OEM and GMP oligo manufacturing.

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.