C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)
C<sub>12</sub>FDG (5-Dodecanoylaminofluorescein Di-&beta;-D-Galactopyranoside)
Citations & References (34)
Invitrogen™

C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)

The β-galactosidase substrate, C12FDG has been covalently modified to include a 12-carbon lipophilic moiety. Once inside the cell, the substrateRead more
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Catalog NumberQuantity
D28935 mg
Catalog number D2893
Price (JPY)
79,100
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Quantity:
5 mg
The β-galactosidase substrate, C12FDG has been covalently modified to include a 12-carbon lipophilic moiety. Once inside the cell, the substrate is cleaved by β-galactosidase producing a fluorescent product that is well retained by the cells, probably by incorporation of the liphophilic tail within the cell membrane. C12FDG is the main component in the ImaGene™ Green kit.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission490⁄514
Quantity5 mg
Shipping ConditionRoom Temperature
SubstrateBeta-Gal Substrate
Detection MethodFluorescence
Substrate PropertiesChemical Substrate
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).

Citations & References (34)

Citations & References
Abstract
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Authors:Noppe G, Dekker P, de Koning-Treurniet C, Blom J, van Heemst D, Dirks RW, Tanke HJ, Westendorp RG, Maier AB,
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to ... More
Optical imaging fiber-based single live cell arrays: a high-density cell assay platform.
Authors:Biran I, Walt DR
Journal:Anal Chem
PubMed ID:12141663
'A high-density, ordered array containing thousands of microwells is fabricated on an optical imaging fiber. Each individually addressable microwell is used to accommodate a single living cell. A charged coupled device (CCD) detector is employed to monitor and spatially resolve the fluorescence signals obtained from each individual cell, allowing simultaneous ... More
An optimized electroporation protocol applicable to a wide range of cell lines.
Authors:Baum C, Forster P, Hegewisch-Becker S, Harbers K
Journal:Biotechniques
PubMed ID:7873174
'A number of transfection methods for mammalian cells are available; however, many cell lines may appear resistant to efficient transfection, or at best, necessitate lengthy optimization procedures in recommended protocols. We describe here an electroporation protocol that yields highly efficient gene transfer (20%-100% of surviving cells) in all 19 cell ... More
Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.
Authors:Patschan S, Chen J, Gealekman O, Krupincza K, Wang M, Shu L, Shayman JA, Goligorsky MS,
Journal:Am J Physiol Renal Physiol
PubMed ID:17928415
'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient ... More
Use of fluorescence-activated cell sorting for rapid isolation of insect cells harboring recombinant baculovirus.
Authors:Sommerfelt MA, Sorscher EJ
Journal:Methods Cell Biol
PubMed ID:7877509
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