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DNA Retardation Gels (6%), 1.0 mm
Invitrogen™

DNA Retardation Gels (6%), 1.0 mm

Novex™ DNA Retardation Gels are 6% polyacrylamide prepared with 0.5X TBE gel buffer, providing clear resolution of fragments used for DNA retardation assays from 60–2500 bp.
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Catalog NumberWells
EC6365BOX10-well
EC63652BOX12-well
EC63655BOX15-well
Catalog number EC6365BOX
Price (JPY)
44,400
Each
Wells:
10-well
Request bulk or custom format
Novex™ DNA Retardation Gels are 6% polyacrylamide prepared with 0.5X TBE gel buffer, providing clear resolution of fragments used for DNA retardation assays from 60–2500 bp. This preparation is good for electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell and are available in 10-, 12-, or 15-well formats.
  • DNA fragments ranging from 60-2,500 bp
  • Prepared with 0.5X TBE as gel buffer
  • Designed to run on the XCell SureLock Mini-Cell
  • Available in 10, 12, and 15 well formats
  • Manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS

To ensure best possible results, these gels are single use and individually wrapped. Detection is performed with ethidium bromide staining of DNA or, for greater sensitivity, with radiolabeling the DNA or protein.

Formulation

Novex DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers

For optimum performance, Novex™ TBE Running Buffer and Novex™ Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex Hi-Density TBE Sample Buffer contains the tracking dyes Bromophenol Blue and Xylene Cyanol as well as the density agent Ficoll™, which yields sharper and straighter bands than conventional density agents.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDNA Retardation Gels, 10-well
For Use With (Equipment)XCell SureLock Mini-Cell
Gel Thickness1 mm
Quantity10 Gels/Box
Sample Buffer TypeTBE Hi-Density Sample Buffer (5X)
Sample TypeDouble-stranded DNA (dsDNA)
Shipping ConditionWet Ice
Gel Percentage6%
Gel TypeDNA Retardation
Product TypeDNA Retardation Gel
Separation Range60 to 2500 bp
Wells10-well
Unit SizeEach
Contents & Storage
• Catalog number refers to a single box of 10 gels

Store at 4°C.

Frequently asked questions (FAQs)

What is a shift assay? What is a supershift assay?

A shift assay is a DNA-binding assay using nondenaturing PAGE. It provides a simple, rapid, and extremely sensitive method for detecting sequence-specific DNA-binding proteins. Proteins bind specifically to an end-labeled DNA fragment corresponding to the individual protein-DNA complexes. You can use the assay to test binding of purified proteins or of uncharacterized factors in crude extracts. This assay also permits quantitative determination of the affinity, abundance, association rate constants, dissociation rate constants, and binding specificity of DNA-binding proteins.

A supershift assay is a variation of the mobility shift DNA-binding assay that uses antibodies to identify proteins present in the protein-DNA complex.Addition of a specific antibody to a binding reaction can have one of several effects. If the protein recognized by the antibody is not involved in complex formation, addition of the antibody should have no effect. If the protein that forms the complex is recognized by the antibody, the antibody can either block complex formation or it can form an antibody-protein-DNA ternary complex and thereby specifically result in a further reduction in the mobility of the protein-DNA complex (a supershift). Results may be different depending upon whether the antibody is added before or after the protein binds DNA (particularly if there are epitopes on the DNA binding surface of the protein).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the difference in composition between the Invitrogen 6% TBE Gels and the Invitrogen 6% DNA Retardation Gels?

The Invitrogen 6% DNA Retardation Gels contain 0.5X TBE. Both gels will work for gel retardation; however, the 1X TBE in the Invitrogen 6% TBE Gels have a higher ionic environment, which may affect DNA-protein interactions. The 0.5X TBE used in the Invitrogen 6% DNA Retardation Gels usually works better, as it offers good fragment separation in electrophoresis yet has an ionic strength low enough to promote DNA-protein interactions.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What's the difference in composition between the 6% TBE gels and the 6% DNA retardation gels?

The 6% DNA retardation gels are 0.5X TBE. Both gels will work for gel mobility shift assays, but the 1X TBE has a higher ionic environment that may affect DNA/protein interactions. 0.5X TBE usually works better.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.