Formaldehyde Fluorescent Detection Kit

The Formaldehyde Detection research-use-only kit is a fluorescent assay designed for the quantification and detection of formaldehyde in human urine and tissue culture media samples.

This complete, ready-to-use kit includes clear 96-well plate(s), formaldehyde standard, formaldehyde detection reagent, and other components to perform the assay. A 96-well fluorescence plate reader capable of reading fluorescent emission at 510 nm, with excitation at 450 nm, is required for use of this kit.

Performance characteristics
• Assay type: fluorescent detection Kit
• Sample types: human urine and tissue culture media samples
• Sensitivity: 0.715 μM
• Standard curve range: 3.1–200 μM
• Reactivity: species independent

Background
Formaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent. Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. This kit was shown to measure formaldehyde in human urine and tissue culture media. Formaldehyde is identical across all species and cell types.

Assay principle
The Formaldehyde Urinary Detection kit designed to quantitatively measure formaldehyde present in tissue culture media and urine samples. A formaldehyde standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a black microtiter plate. The fluorescent reaction is initiated with the formaldehyde detection reagent, which is pipetted into each well. After a short incubation the emission of the generated fluorescent signal is detected in a microtiter plate reader capable of measuring 510 nm fluorescence utilizing 450 nm excitation wavelength.

Related links
Learn more about ELISA kits
Learn more about other immunoassays