Cfr10I (BsrFI) (10 U/μL)
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Thermo Scientific™

Cfr10I (BsrFI) (10 U/μL)

5' R ↓C C G G Y 3' 3' Y G G C C ↑R 5' Thermo Scientific Cfr10I (BsrFI)Read more
Catalog number ER0181
Price (JPY)/ 200 units
Price:13,000
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5'  R ↓C  C  G  G  Y   3' 
3'  Y  G  G  C  C ↑R   5' 

Thermo Scientific Cfr10I (BsrFI) restriction enzyme recognizes R^CCGGY sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Bse118I, BsrFI, BssAIm). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 U/μL
Methylation SensitivityCpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
EnzymeCfr10I (BsrFI)
Compatible BufferUnique Buffer (10X Buffer Cfr10I (BsrFI))
Sensitive to Heat InactivationNo
Optimal Reaction Temperature37°C
Type IIS RENo
Quantity200 units
Product TypeRestriction Enzyme
Research CategoryTraditional Cloning
Unit Size200 units