FocalCheck™ Fluorescence Microscope Test Slide #2, for spectral imaging systems
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Invitrogen™
FocalCheck™ Fluorescence Microscope Test Slide #2, for spectral imaging systems
The FocalCheck™ fluorescence microscope test slide #2 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filterRead more
The FocalCheck™ fluorescence microscope test slide #2 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filter performance. This slide provides a robust, reproducible method of evaluating the performance of spectral imaging systems, as well as the ability to discriminate closely overlapping spectra. The slide consists of 6 μm-diameter microspheres labeled with a series of spectrally overlapping dyes.
Quality assessment of confocal microscopy slide-based systems: instability.
Authors:Zucker RM
Journal:Cytometry A
PubMed ID:16807898
'BACKGROUND: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. METHODS: A number of tests have been devised to evaluate equipment performance and instability. The following four instability ... More
Reliability of confocal microscopy spectral imaging systems: use of multispectral beads.
Authors:Zucker RM, Rigby P, Clements I, Salmon W, Chua M,
Journal:Cytometry A
PubMed ID:17266146
BACKGROUND: There is a need for a standardized, impartial calibration, and validation protocol on confocal spectral imaging (CSI) microscope systems. To achieve this goal, it is necessary to have testing tools to provide a reproducible way to evaluate instrument performance. METHODS: We evaluated fluorescent spectral beads (FocalCheck) from Molecular Probes/Invitrogen ... More
Snapshot hyperspectral imaging augments pixel dwell time and acquisition speeds over existing scanning systems, making it a powerful tool for fluorescence microscopy. While most snapshot systems contain fixed datacube parameters (x,y,?), our novel snapshot system, called the lenslet array tunable snapshot imaging spectrometer (LATIS), demonstrates tuning its average spectral resolution ... More