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Human Plateable Hepatocytes, Transporter Qualified

Human Plateable Hepatocytes, Transporter Qualified assessed for transporter function using uptake transporter assays at both 4°C and 37°C.

Catalog Number	Quantity
HMCPTS	1.5 mL vial

Catalog number HMCPTS

Price (JPY)

Quantity:

1.5 mL vial

Plateable cryopreserved human hepatocytes assessed for transporter function using uptake transporter assays at both 4°C and 37°C.

Cells per vial: 4-8 million

Hepatocyte cultures were incubated with taurocholate and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP/BSEP and OATPs/MRP2. Accumulation rates and facilitated active uptake were calculated.

Get the Results You Need with GIBCO Hepatocytes

High quality hepatocytes, with high viabilities, proper cell morphology, and transporter function increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

Viability ≥ 70%
Attachment efficiency
Monolayer formation and integrity
Confluency >80% on Day 4
Tested for plating in 96 well format
Phase I enzyme activities
Transporter activity
Bile canaliculi formation
Relevant CYP450 polymorphisms

Note:

These products require shipment in LN₂ vapor dewars therefore additional fees may apply.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Donor SourceSingle Donor

No. of Cells4-8 x 10⁶

Product LineGibco

Product TypeHepatocytes

Quantity1.5 mL vial

Cell LinePrimary

Cell TypeHepatocytes

Culture TypeHepatocyte Cell Culture

FormCryopreserved

SpeciesHuman

TissueLiver

Unit SizeEach

Contents & Storage

Store in a cryostorage dewar or -135°C freezer.

Frequently asked questions (FAQs)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

What should I plate hepatocyte cells on?

Hepatocytes need to be plated on collagen I-coated culture vessels.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Human Plateable Hepatocytes, Transporter Qualified FAQs