Denhardt's solution (50X)
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Denhardt's solution (50X)
Thermo Scientific Chemicals

Denhardt's solution (50X)

Denhardt's solution (50X) is a mixture of blocking agents used in membrane-based hybridization protocols.
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50 mL
100 mL
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Catalog number J63135.AE
also known as J63135-AE
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Quantity:
100 mL
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Specifications
Chemical Name or MaterialDenhardt's solution
Concentration50X
MDL NumberMFCD00717549
Physical FormLiquid
Recommended StorageStore at -20°C
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Denhardt's solution is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • Formulated in 1966, Denhardt's solution is a combination of high-molecular weight polymers used to prevent non-specific binding of nucleic acid probes to membranes and filters
  • Denhardt's solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin

Application

  • Denhardt's solution is used in Northern and Southern blotting to prevent non-specific binding of detection reagents to membranes
  • It can be used along with gelatin-chrome alum in in situ hybridization assays to maintain chromosome integrity
  • It has been used to facilitate quantitative DNA hybridization on nylon membranes
RUO – Research Use Only

General References:

  1. D. Denhardt, 1966, Biochem. Biophys. Res. Commun. 23, 641-646.
  2. Lee GM, Rasch EM, Musich PR. Improved in situ hybridization and G-banding by pretreatment with Denhardt's solution and gelatin-chrome alum. Histochem J. 1985 Nov;17(11):1221-33. doi: 10.1007/BF01002504. PMID: 2417994.
  3. Cannon G, Heinhorst S, Weissbach A. Quantitative molecular hybridization on nylon membranes. Anal Biochem. 1985 Aug 15;149(1):229-37. doi: 10.1016/0003-2697(85)90500-7. PMID: 4073479.