S.N.A.P.™ Plasmid DNA MidiPrep Kit
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Invitrogen™

S.N.A.P.™ Plasmid DNA MidiPrep Kit

The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmidRead more
Catalog number K191001
Price (JPY)/ 20 preps
Price:102,900
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The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:

• Lyse cells and filter debris with the S.N.A.P.™ filtering column
• Apply flow-through to the S.N.A.P.™ DNA binding column
• Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE buffer

Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Isolation TechnologySpin Column
Sample TypeBacterial Culture
ScaleMidi
Final Product TypePlasmid DNA
For Use With (Application)Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Prep Scale100-200 μg (Medium-Scale) Plasmid DNA
Product LineS.N.A.P. ™
Product TypePlasmid DNA MidiPrep Kit
Quantity20 preps
TargetPlasmid DNA
Test Time40 min.
Unit Size20 preps
Contents & Storage
Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.