Fluorescein isothiocyanate, isomer 1, 95%

Name: Fluorescein isothiocyanate, isomer 1, 95%
SKU: L09319.MD

CAS: 3326-32-7 | C21H11NO5S | 389.381 g/mol

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Catalog number L09319.MD

also known as L09319-MD

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Quantity:

250 mg

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Chemical Identifiers

CAS3326-32-7

IUPAC Name3',6'-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one

Molecular FormulaC21H11NO5S

InChI KeyMHMNJMPURVTYEJ-UHFFFAOYSA-N

SMILESOC1=CC=C2C(OC3=CC(O)=CC=C3C22OC(=O)C3=CC(=CC=C23)N=C=S)=C1

Specifications

Specification Sheet

FormPowder

Identification (FTIR)Conforms

Appearance (Color)Orange to brown

Assay (HPLC)≥94.0%

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

Solubility
Slightly soluble in DMSO, ethanol, methanol DMF and acetone

Notes
Incompatible with strong oxidizing agents, strong bases and amines.

RUO – Research Use Only

General References:

Reagent for fluorescent labelling of proteins: Anal. Biochem., 20, 178 (1968).
Guilini, C.; Baehr, C.; Schaeffer, E.; Gizzi, P.; Rufi, F.; Haiech, J.; Weiss, E.; Bonnet, D.; Galzi, J. New Fluorescein Precursors for Live Bacteria Detection. Anal. Chem. 2015, 87 (17), 8858-8866.
Chen, Y.; Yang, C.; Li, T.; Zhang, M.; Liu, Y.; Gauthier, M. A.; Zhao, Y.; Wu, C. The Interplay of Disulfide Bonds, α-Helicity, and Hydrophobic Interactions Leads to Ultrahigh Proteolytic Stability of Peptides. Biomacromolecules 2015, 16 (8), 2347-2355.