Fluorescein-12-dUTP Solution (1 mM)

Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.

Fluorescein-12-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase
• phi29 DNA Polymerase
• Klenow Fragment, exo-
• Klenow Fragment
• DNA Polymerase I

Applications
• Enzymatic non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random primed labeling
• Primer extension