ReadyProbes™ Reagent F-Actin Phalloidin Conjugates

ActinGreen and ActinRed ReadyProbes Reagents are selective, room temperature-stable solutions, with high-affinity F-actin probe conjugated to bright, photostable, fluorescent dyes. ActinGreen 488 reagent uses bright Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 reagent uses Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle. ActinRed 568 uses Cell Paint’s Alexa Fluor™ 568 Phalloidin (Cat. No. A12380), and ActinRed 647 Plus uses the very bright Alexa Fluor™ Plus 647 Phalloidin (Cat. No. A30107) both packaged in a dual-use dropper bottle.

ActinGreen and ActinRed ReadyProbes Reagents are selective, room temperature-stable solutions, with high-affinity F-actin probe conjugated to bright, photostable, fluorescent dyes. ActinGreen 488 reagent uses bright Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 reagent uses Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle. ActinRed 568 uses Cell Paint's Alexa Fluor™ 568 Phalloidin (Cat. No. A12380), and ActinRed 647 Plus uses the very bright Alexa Fluor™ Plus 647 Phalloidin (Cat. No. A30107) both packaged in a dual-use dropper bottle.

These ready-to-use solutions combine excellent brightness with exceptional F-actin staining. Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding or binding to G-actin.

Features

• High-affinity staining of F-actin with exceptional specificity compared to antibody methods
• ActinGreen 488 Excitation/Emission: (495/518 nm), detected through standard GFP and FITC filter sets
• ActinRed 555 Excitation/Emission: (540/565 nm), detected through standard RFP and TRITC filters sets
• ActinRed 568 Excitation/Emission: (578/600 nm), detected through standard RFP and TRITC filters sets
• ActinRed 647 Plus Excitation/Emission: (650/668 nm), detected through standard Texas Red and Cy5 filters sets
• Use counterstain (i.e., DAPI or NucBlue ReadyProbes reagent) as desired

ReadyProbes format advantages

• Ready-to-use formulation - no need to dilute, weigh, or pipette
• Just use two drops (80 μL) per mL of medium to stain, incubate 30 minutes, wash, then image
• ActinRed 568 ReadyProbes Reagent and ActinRed 647 Plus ReadyProbes Reagent are supplied in a dual-use bottle that supports either direct pipetting for plate-based workflows or dropwise dispensing after installation of the included dropper tip and cap.
• Stable at room temperature—keep handy on the benchtop

Applications

• For fixed and permeabilized cells or tissue
• Fluorescence microscopy (IF/IHC/ICC)
• High content screening, high content imaging
• Flow cytometry

Suggestions for use

• All ActinRed and ActinGreen reagents may be added directly to fixed cells in full medium or buffer solutions

In most cases 2 drops/mL and an incubation time of 15–30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.