Ni-NTA Agarose

Citations & References (4)

Invitrogen™

Ni-NTA Agarose

Name: Ni-NTA Agarose
SKU: R90101

Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.Read more

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Catalog Number	Quantity
R90101	10 mL
R90115	25 mL
R90110	100 mL

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Catalog number R90101

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Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine. One-step purification can be performed under both native and denaturing conditions. Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity10 mL

Stationary PhaseNi-NTA

Column TypeAffinity Column

FormSuspension

TypeAgarose

Unit SizeEach

Contents & Storage

The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (4)

Citations & References

Abstract

A protein kinase encoded by the t complex responder gene causes non-mendelian inheritance

Authors:BERNHARD G. HERRMANN, BIRGIT KOSCHORZ, KARIN WERTZ, K. JOHN MCLAUGHLIN* & ANDREAS KISPERT

Journal:Nature

PubMed ID:10647005

'Males heterozygous for the t-haplotype form of mouse chromosome 17 preferentially transmit the t-chromosome to their progeny. Several distorter/sterility loci carried on the t-haplotype together impair flagellar function in all spermatozoa whereas the responder, Tcr, rescues t-sperm but not wild-type sperm. Thus, t-sperm have anadvantage over wild-type sperm in fertilizing ... More

DNA Polymerase lambda, a Novel DNA Repair Enzyme in Human Cells.

Authors: Garcia-Diaz Miguel; Bebenek Katarzyna; Sabariegos Rosario; Dominguez Orlando; Rodriguez Josana; Kirchhoff Tomas; Garcia-Palomero Esther; Picher Angel J; Juarez Raquel; Ruiz Jose F; Kunkel Thomas A; Blanco Luis;

Journal:J Biol Chem

PubMed ID:11821417

'DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5''-deoxyribose-5-phosphate lyase activity in pol lambda supports a function of this enzyme in base excision repair. However, the ... More

A ribozyme that ligates RNA to protein.

Authors: Baskerville Scott; Bartel David P;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12077317

'We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5'' terminus of an RNA and a specific polypeptide. This reaction differs from that of previously identified ribozymes, although the product is analogous to the enzyme-nucleotidyl intermediates ... More

Transduction of MIN6 beta Cells with TAT-Syntaxin SNARE Motif Inhibits Insulin Exocytosis in Biphasic Insulin Release in a Distinct Mechanism Analyzed by Evanescent Wave Microscopy.

Authors: Ohara-Imaizumi Mica; Nakamichi Yoko; Nishiwaki Chiyono; Nagamatsu Shinya;

Journal:J Biol Chem

PubMed ID:12393909

To investigate the in vivo interaction of syntaxin-mediated soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) assembly and insulin exocytosis in biphasic release, we examined the dynamics of insulin granule motion such as docking and fusion with the plasma membrane when the syntaxin SNARE motif (H3 domain) was transduced into ... More