Streptavidin, Alexa Fluor™ 430 conjugate
Streptavidin, Alexa Fluor™ 430 conjugate
Invitrogen™

Streptavidin, Alexa Fluor™ 430 conjugate

Our Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm). Streptavidin, a nonglycosylated biotin-binding proteinRead more
Catalog NumberQuantity
S11237
also known as S-11237
1 mg
Catalog number S11237
also known as S-11237
Price (JPY)
67,200
Each
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Quantity:
1 mg
Our Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm). Streptavidin, a nonglycosylated biotin-binding protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission434/539
For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Label or DyeAlexa Fluor Dyes
Product TypeStreptavidin Conjugate (Fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 430
FormSolid
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
Contains 1 vial of streptavidin Alexa Fluor™ 430 conjugate.

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (1)

Citations & References
Abstract
Detection and quantification of biotinylated proteins using the Storm 840 Optical Scanner.
Authors:Lewis B, Rathman S, McMahon RJ
Journal:J Nutr Biochem
PubMed ID:12770643
'The use of the avidin-biotin interaction is becoming an increasingly common method for the detection of proteins. The use of fluorescence detection with avidin-biotin systems has the potential to greatly increase both the sensitivity and linearity of this type of analysis. In this report, three fluorescent systems were tested for ... More