SlowFade™ Gold Antifade Mountant

Citations & References (7)

Invitrogen™

SlowFade™ Gold Antifade Mountant

Name: SlowFade™ Gold Antifade Mountant
SKU: S36936

SlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides.Read more

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Catalog Number	Quantity
S36936	10 mL
S36937	5 x 2 mL
S36940	2 mL

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Catalog number S36936

Price (JPY)

31,600

Each

Quantity:

10 mL

SlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. SlowFade Gold Antifade Mountant is glycerol-based, with no mixing or curing required, which makes it ideal for immediate viewing of the sample. It comes ready-to-use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

Key attributes:
• Protects dyes from fading during imaging
• Ready-to-use liquid, ideal for immediate sample viewing
• Mounted samples are stable for weeks
• Maintains signal strength—little to no quenching
• Ideal for Alexa Fluor dyes

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

SlowFade Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes in cultured cells or thin tissue slices, SlowFade Diamond Antifade Mountant is recommended. For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 μm, try SlowFade Glass Antifade Mountant.

Select best SlowFade antifade reagent for your experimental needs ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Green FeaturesLess hazardous, sustainable packaging

Product LineSlowFade

Quantity10 mL

Shipping ConditionRoom Temperature

For Use With (Equipment)Microscope

FormLiquid

Product TypeMounting Media

Solution TypeAnti-fade Mountant

Unit SizeEach

Contents & Storage

Storage at room temperature is recommended but can also be stored frozen (-5° to -30°C). Protect from light.

Frequently asked questions (FAQs)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (7)

Citations & References

Abstract

Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.

Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K

Journal:Am J Physiol Renal Physiol

PubMed ID:16159901

'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More

Identification and characterization of small molecules that inhibit intracellular toxin transport.

Authors:Saenz JB, Doggett TA, Haslam DB

Journal:Infect Immun

PubMed ID:17576758

'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins ... More

Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.

Authors:Fumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,

Journal:Mol Pharm

PubMed ID:19492848

We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More

Programmable in situ amplification for multiplexed imaging of mRNA expression.

Authors:Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA,

Journal:Nat Biotechnol

PubMed ID:21037591

In situ hybridization methods enable the mapping of mRNA expression within intact biological samples. With current approaches, it is challenging to simultaneously map multiple target mRNAs within whole-mount vertebrate embryos, representing a significant limitation in attempting to study interacting regulatory elements in systems most relevant to human development and disease. ... More

A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining.

Authors:Wu JS, Luo L

Journal:Nat Protoc

PubMed ID:17487202

This protocol describes a basic method for dissection and immunofluorescence staining of the Drosophila brain at various developmental stages. The Drosophila brain has become increasingly useful for studies of neuronal wiring and morphogenesis in combination with techniques such as the 'mosaic analysis with a repressible cell marker' (MARCM) system, where ... More

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