TetraSpeck™ Microspheres, 4.0 μm, fluorescent blue/green/orange/dark red
TetraSpeck™ Microspheres, 4.0 μm, fluorescent blue/green/orange/dark red
Invitrogen™

TetraSpeck™ Microspheres, 4.0 μm, fluorescent blue/green/orange/dark red

The 4.0 μm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separatedRead more
Catalog NumberQuantity
T7283
also known as T-7283
0.5 mL
Catalog number T7283
also known as T-7283
Price (JPY)
75,500
0.5 mL
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Quantity:
0.5 mL
The 4.0 μm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.

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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibration TypeConfocal Microscope Calibration, Fluorescence Microscope Calibration
FormatSuspension Beads
Product LineTetraSpeck
Quantity0.5 mL
Shipping ConditionRoom Temperature
ColorOrange, Dark Red, Blue, Green
Diameter (Metric)4 μm
Product TypeMicrosphere
Unit Size0.5 mL
Contents & Storage
Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

What are the excitation/emission peaks for TetraSpeck Microspheres?

The TetraSpeck Microspheres (Cat. Nos. T7279, T7280, T7281, T7283, T7284, T14792) are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks at 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).

TetraSpeck Blue Dye Spectra
Fluorescence excitation and emission spectra of bead encapsulated TetraSpeck blue dye.
TetraSpeck Blue Dye Spectra

TetraSpeck Orange Dye Spectra


TetraSpeck Green Dye Spectra
TetraSpeck Green Dye Spectra

TetraSpeck Dark Red Dye Spectra
TetraSpeck Dark Red Spectra

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (14)

Citations & References
Abstract
The cohesion protein ORD is required for homologue bias during meiotic recombination.
Authors:Webber HA, Howard L, Bickel SE
Journal:J Cell Biol
PubMed ID:15007062
'During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along ... More
Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level.
Authors:Ober RJ, Martinez C, Lai X, Zhou J, Ward ES
Journal:Proc Natl Acad Sci U S A
PubMed ID:15258288
'IgG transport within and across cells is essential for effective humoral immunity. Through a combination of biochemical and in vivo analyses, the MHC class I-related neonatal Fc receptor (FcRn) is known to play a central role in delivering IgGs within and across cells. However, little is known about the molecular ... More
Intensity calibration and automated cell cycle gating for high-throughput image-based siRNA screens of mammalian cells.
Authors:Poon SS, Wong JT, Saunders DN, Ma QC, McKinney S, Fee J, Aparicio SA,
Journal:Cytometry A
PubMed ID:18698634
'High-content microscopic screening systems are powerful tools for extracting quantitative multiparameter measures from large number of cells under numerous conditions. These systems perform well in applications that monitor the presence of objects, but lack in their ability to accurately estimate object intensities and summarize these findings due to variations in ... More
Practical confocal microscopy and the evaluation of system performance.
Authors:Zucker RM, Price OT
Journal:Methods
PubMed ID:10491274
'The laser scanning confocal microscope has enormous potential in many fields of biology. Currently there is a subjective nature in the assessment of a confocal microscope''s performance by primarily evaluating the system with a specific test slide provided by the user''s laboratory. To achieve better performance from the equipment, it ... More
Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly.
Authors:Puri N, Kruhlak MJ, Whiteheart SW, Roche PA
Journal:J Immunol
PubMed ID:14607937
'Mast cells possess specialized granules that, upon stimulation of surface FcR with IgE, fuse with the plasma membrane, thereby releasing inflammatory mediators. A family of membrane fusion proteins called SNAREs, which are present on both the granule and the plasma membrane, plays a role in the fusion of these granules ... More