pAd/PL-DEST™ Gateway™ Vector Kit

Catalog Number	Quantity
V49420	6 μg

Catalog number V49420

Price (JPY)

300,700

Quantity:

The pAd⁄PL-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Allows gene of interest to be controlled by a promoter of choice
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway™ Technology for efficient and rapid cloning
• Promoterless vector that allows gene of interest to be controlled by a promoter of choice
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Ampicillin selection marker

Kit includes
• pAd⁄PL-DEST™ Vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible SystemConstitutive

Delivery TypeAdenoviral

For Use With (Application)Clone your own Promoter, Viral Expression

Product TypeAdenovirus Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)None

VectorpDEST

Cloning MethodGateway

Product LineGateway, ViraPower

PromoterNone (Promoterless)

Protein TagUntagged

Unit Size6 µg

Contents & Storage

• pAd⁄PL-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pAd/PL-DEST™ Gateway™ Vector Kit FAQs

Citations & References (1)

Citations & References

Abstract

Pulmonary interleukin-23 gene delivery increases local T-cell immunity and controls growth of Mycobacterium tuberculosis in the lungs.

Authors:Happel KI, Lockhart EA, Mason CM, Porretta E, Keoshkerian E, Odden AR, Nelson S, Ramsay AJ,

Journal:Infect Immun

PubMed ID:16113296

Interleukin-23 (IL-23) is a heterodimeric cytokine that shares IL-12 p40 but contains a unique p19 subunit similar to IL-12 p35. Previous studies indicate a greater importance for intact IL-12/23 p40 expression than IL-12 p35 for immunity against Mycobacterium tuberculosis, suggesting a role for IL-23 in host defense. The effects of ... More