WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:
• High specificity, clean background • Ultra-sensitivity-femtogram levels detectable • Long-lasting signals-up to 5 days • Results in less than 3 hours • Permanent photographic image
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Reactivity
Mouse
Shipping Condition
Wet Ice
Substrate Type
AP (Alkaline Phosphatase) Substrate
Detection Method
Chemiluminescent
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
WesternBreeze™
Product Type
Western Blot Kit
Cross Reactivity
Mouse
Contents & Storage
The WesternBreeze™ Chemiluminescent Kits include blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution (anti-mouse, anti-rabbit or anti-goat), ready-to-use chemiluminescent substrate, wash solutions, incubation trays, pre-cut filter papers, polyester sheet for even substrate development on the membrane. Each kit contains complete reagents for 20 blots. Store the kits at +4°C. Guaranteed stable for 6 months when properly stored.