ZOOM™ Carrier Ampholytes 4-6, 10mL

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectricRead more

Catalog Number	Quantity
ZM0025	10 mL

Catalog number ZM0025

Price (JPY)

48,100

10 mL

Quantity:

10 mL

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectric points in an electric field, thereby establishing a pH gradient in solution or contributing to the gradient when used with IPG strips. Carrier ampholytes help stabilize the pH gradient and current in IPG strips and aid in protein solubility, resulting in reproducible IEF resolution.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product LineZOOM

Product TypeCarrier Ampholyte

Quantity10 mL

Shelf Life1 Year

Shipping ConditionRoom Temperature

pH Range4 to 6

Unit Size10 mL

Contents & Storage

ZOOM™ Carrier Ampholytes are supplied as 10 ml bottles. Store at +4°C. Guaranteed stable for 1 year when properly stored.

Frequently asked questions (FAQs)

When running the ZOOM IEF Fractionator, if there is not a solubility issue, will the proteins/peptides in the sample migrate to their expected chambers based on isoelectric point in the absence of carrier ampholytes? Won't they act as ampholytes within each chamber?

Proteins are ampholytes, but in general they are poor carrier ampholytes. The profiles of the fractions are similar, but not identical, to those obtained with ampholytes in the sample.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I heat my protein sample prepared using ZOOM 2D Protein Solubilizer?

We do not recommend heating protein samples containing urea over 37 degrees C as elevated temperatures cause urea to hydrolyze to isocyanate, which modifies proteins by carbamylation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How do you recommend storing protein samples prepared in the Sample Rehydration buffer?

We recommend storing them at -80 degrees C. We do not recommend storing them at -20 degrees C

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much ampholyte do you recommend adding to the sample rehydration buffer?

The recommended ampholyte concentration in the sample rehydration buffer is 0.5%.

*If you are loading 5-50 µg of protein (pure protein or crude lysate) per ZOOM strip, use 0.5% ampholytes in the sample rehydration buffer.
*If you are loading >50 µg of protein (crude lysate or fractionated sample) per ZOOM Strip, use 0.5-2% ampholytes in the sample rehydration buffer.

Note: Higher ampholyte concentration requires longer focusing times.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the maximum recommended volume of protein sample to be added to the Sample Rehydration buffer?

The maximum volume of the protein sample should at most be 1/6 of the final sample volume that will be added to the strip. A good general volume would be 5-10µL. 140 µL of sample diluted in Sample Rehydration buffer is used to rehydrate each ZOOM Strip for the standard rehydration time of one hour. For overnight rehydration, we recommend using 155 µL of diluted sample.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all ZOOM™ Carrier Ampholytes 4-6, 10mL FAQs