BMH (bismaleimidohexane)

Citas y referencias (4)

Thermo Scientific™

BMH (bismaleimidohexane)

Thermo Scientific Pierce BMH es un entrecruzador de maleimida de brazo largo para conjugación covalente e irreversible entre grupos sulfhidriloMás información

Número de catálogo	Cantidad
22330	50 mg

Número de catálogo 22330

Precio (MXN)

Cantidad:

50 mg

Pedido a granel o personalizado

Thermo Scientific Pierce BMH es un entrecruzador de maleimida de brazo largo para conjugación covalente e irreversible entre grupos sulfhidrilo (por ejemplo, cisteínas de proteínas o péptidos).

Características de BMH:

• Grupos reactivos: maleimida (ambos extremos)
• Reactivo a: grupos sulfhidrilo
• Entrecruzador sulfhidrilo a sulfhidrilo largo (13,0 Å) compuesto por grupos de maleimida y un brazo espaciador de 11 átomos
• Insoluble al agua: disolver primero en DMF o DMSO y añadir a los tampones de reacción acuosa
• No escindible

Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

Productos relacionados
BMB (1,4-bismaleimidobutano)
BMOE (bismaleimidoetano)
BM(PEG)2 (1,8-bismaleimido-dietilenglicol)
BM(PEG)3 (1,11-bismaleimido-trietilenglicol)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Permeabilidad celularSí

DescripciónBMH

FormularioPolvo

Método de etiquetadoEtiqueta química

Peso molecular276.29

PegiladoNo

Línea de productosPierce

Cantidad50 mg

Fracción reactivaMaleimida

Condiciones de envíoAmbiente

SolubilidadDMF, DMSO

Longitud del brazo del separador13,0 Å

Soluble en aguaNo

Reactividad químicaSulfhidrilo-sulfhidrilo

CleavableNo

Tipo de enlace cruzadoHomobifuncionales

FormatoEstándar

Tipo de productoEntrecruzador

SeparadorMedio (de 10 a 30 Å)

Unit SizeEach

Contenido y almacenamiento

Tras su recepción, almacenar a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for BMH (bismaleimidohexane)?

BMH (bismaleimidohexane) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (4)

Citations & References

Abstract

Membrane curvature induced by Arf1-GTP is essential for vesicle formation.

Authors:Beck R, Sun Z, Adolf F, Rutz C, Bassler J, Wild K, Sinning I, Hurt E, Brügger B, Béthune J, Wieland F

Journal:Proc Natl Acad Sci U S A

PubMed ID:18689681

'The GTPase Arf1 is considered as a molecular switch that regulates binding and release of coat proteins that polymerize on membranes to form transport vesicles. Here, we show that Arf1-GTP induces positive membrane curvature and find that the small GTPase can dimerize dependent on GTP. Investigating a possible link between ... More

The MUC1-C oncoprotein binds to the BH3 domain of the pro-apoptotic BAX protein and blocks BAX function.

Authors:Ahmad R, Alam M, Rajabi H, Kufe D

Journal:J Biol Chem

PubMed ID:22544745

'The pro-apoptotic BAX protein contains a BH3 domain that is necessary for its dimerization and for activation of the intrinsic apoptotic pathway. The MUC1 (mucin 1) heterodimeric protein is overexpressed in diverse human carcinomas and blocks apoptosis in the response to stress. In this study, we demonstrate that the oncogenic ... More

Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis.

Authors:Kida Y, Morimoto F, Sakaguchi M

Journal:J Cell Biol

PubMed ID:18166653

During protein integration into the endoplasmic reticulum, the N-terminal domain preceding the type I signal-anchor sequence is translocated through a translocon. By fusing a streptavidin-binding peptide tag to the N terminus, we created integration intermediates of multispanning membrane proteins. In a cell-free system, N-terminal domain (N-domain) translocation was arrested by ... More

Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation.

Authors:Gogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D

Journal:J Biol Chem

PubMed ID:21712378

Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated translocation of Bax to mitochondria where it underwent oligomerization to initiate apoptosis. Resveratrol treatment promoted ... More