FastDigest NotI
FastDigest NotI
Thermo Scientific™

FastDigest NotI

5' G C ↓G G C C G C 3' 3' C G C C G G ↑C G 5'深入閱讀
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產品號碼Quantity
FD059450 μL (50 Reactions)
FD059320 μL (20 Reactions)
FD0596250 μL (250 Reactions)
FD0595150 μL (150 Reactions)
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產品號碼 FD0594
價格 (TWD)/ 50 reactions
價格3,080.00
線上優惠2,780.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
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50 μL (50 Reactions)
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價格 (TWD)/ 50 reactions
線上優惠2,780.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
新增至購物車
FastDigest NotI
產品號碼FD0594
價格 (TWD)/ 50 reactions
線上優惠2,780.00僅限網絡訂單。不包括供應中心
(ends 01-Jan-2025)
-
新增至購物車
5'  G  C ↓G  G  C  C  G  C   3' 
3'  C  G  C  C  G  G ↑C  G   5' 

Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: CciNI.

Thermo Scientific FastDigest NotI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
規格
Methylation SensitivityCpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
EnzymeNot I
Compatible Buffer10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat InactivationYes
Optimal Reaction Temperature37°C
Type IIS RENo
Quantity50 μL (50 Reactions)
Product TypeRestriction Enzyme
Research CategoryTraditional Cloning
Product LineFastDigest™
Overhang5' protruding ends
Inactivation Temperature80°C
Unit Size50 reactions
內容物與存放
• FastDigest NotI (50 μL)
• 10X FastDigest Buffer (1.0 mL)
• 10X FastDigest Green Buffer (1.0 mL)

Store at –20°C.