Thermo Scientific™

T4 DNA Polymerase (5 U/µL)

Catalog number:  EP0061

Related applications:


| PCR Cloning

| Restriction Enzyme Cloning

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Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.


Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment
• Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers


• Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs(see​ References1, 2)
• Blunting of PCR products with 3'-dA overhangs
• Synthesis of labeled DNA probes by the replacement reaction(see​ Reference3)
• Oligonucleotide-directed site-specific mutagenesis(see​ Reference4)
• Ligation-independent cloning of PCR products
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: T4 DNA Polymerase
Quantity: 100 unit
Includes: 1mL of 5X Reaction Buffer