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Invitrogen

FOXP3 Monoclonal Antibody (eBio7979 (221D/D3)), eBioscience™

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12 Published Figures
37 References
View all (164) FOXP3 antibodies

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Product Details

14-7979-82

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 17 publications 17 publications

Immunohistochemistry (IHC)

-
View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 3 publications 3 publications

Immunoprecipitation (IP)

-
View 1 publication 1 publication

ChIP assay (ChIP)

-
View 1 publication 1 publication

Proximity Ligation Assay (PLA) (PLA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Dog, Human, Mouse, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

eBio7979 (221D/D3)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468500

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human and mouse Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to human Foxp3 include PCH101 (Product # 72-5776) and 236A/E7 (Product # 12-4777) . Additional antibodies to mouse Foxp3 include FJK-16s (Product # 72-5775) and NRRF-30 (Product # 12-4771) .

The eBio7979 antibody reacts with human and mouse foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.

Applications Reported: This eBio7979 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This eBio7979 antibody has been tested by immunoblotting of normal human peripheral blood mononuclear cell lysates at 1-2 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: forkhead box P3; forkhead box P3 transcript variant; Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; JM2; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy; unnamed protein product

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; scurfin; sf; XPID

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UniProt ID: (Human) Q9BZS1, (Mouse) Q99JB6

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Entrez Gene ID: (Human) 50943, (Mouse) 20371

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding zinc ion binding histone acetyltransferase binding identical protein binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding sequence-specific double-stranded DNA binding core promoter binding nucleic acid binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter B cell homeostasis negative regulation of cytokine production myeloid cell homeostasis CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment T cell mediated immunity tolerance induction tolerance induction to self antigen T cell tolerance induction regulation of immunoglobulin production positive regulation of T cell tolerance induction regulation of T cell anergy positive regulation of T cell anergy negative regulation of chronic inflammatory response negative regulation of T cell cytokine production positive regulation of peripheral T cell tolerance induction T cell anergy chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response negative regulation of cell proliferation response to virus gene expression positive regulation of gene expression negative regulation of gene expression establishment of endothelial blood-brain barrier negative regulation of NF-kappaB transcription factor activity response to lipopolysaccharide negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-17 production negative regulation of interleukin-2 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-4 production negative regulation of CREB transcription factor activity positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation transforming growth factor beta1 production positive regulation of transforming growth factor beta1 production immature T cell proliferation in thymus positive regulation of immature T cell proliferation in thymus CD4-positive, alpha-beta T cell proliferation T cell proliferation T cell activation negative regulation of T cell proliferation T cell homeostasis CD4-positive, alpha-beta T cell differentiation positive regulation of CD4-positive, alpha-beta T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulatory T cell differentiation positive regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of activated T cell proliferation alpha-beta T cell proliferation negative regulation of alpha-beta T cell proliferation lymphocyte proliferation isotype switching to IgE isotypes negative regulation of isotype switching to IgE isotypes regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response T cell receptor signaling pathway response to rapamycin negative regulation of T-helper 17 cell differentiation negative regulation of CD4-positive, alpha-beta T cell proliferation cytokine production anatomical structure morphogenesis negative regulation of histone deacetylation positive regulation of histone acetylation negative regulation of histone acetylation negative regulation of cytokine biosynthetic process negative regulation of interferon-gamma biosynthetic process negative regulation of interleukin-2 biosynthetic process negative regulation of cytokine secretion
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