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FIGURE: 1 / 5
Mouse IgG Secondary Antibody (A56580) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Mouse IgG, Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A56580, 1 µg/mL) was performed on fixed and permeabilized HeLa cells stained with Mouse beta Tubulin Monoclonal Antibody (2 28 33) (Product # 32-2600, 1:500). Panel a) shows representative cells that were stained for detection and localization of beta Tubulin protein, Panel b) is stained fo... View More
Product Details
A56580
Applications
Tested Dilution
Western Blot (WB)
1-2 µg/mL
Immunocytochemistry (ICC/IF)
0.5-4 µg/mL
Flow Cytometry (Flow)
1-4 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
35H35L37
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.5
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity and superior lot-to-lot consistency.
Minimal cross-reactivity with Human, Goat, Donkey, Guinea Pig, Horse and Bovine IgG observed.
This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.
Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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