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Invitrogen
Synaptotagmin 1 Monoclonal Antibody (ASV48)
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FIGURE: 1 / 2
Synaptotagmin 1 Antibody (MA1-25482) in WB
Western blot was performed using Anti-Synaptotagmin 1 Monoclonal Antibody (ASV48) (Product # MA1-25482) and a 60kDa band corresponding to Synaptotagmin 1 was observed across tissues tested . Whole cell extracts (30 µg lysate) of Mouse Ovary (Lane 1), Rat Ovary (Lane 2), Mouse Retina (Lane 3), Mouse Brain (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The... View More
Product Details
MA1-25482
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Mouse,
Non-human primate,
Rat,
Shark
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
ASV48
Immunogen
Rat brain synaptic junction protein complexes.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Possible positive controls for this product are rat brain tissue extract.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin's Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin XI; Synaptotagmin-1; Synaptotagmin-2; SYT2; SytI; SytII
Gene Aliases: AW124717; G630098F17Rik; P65; SYT1; SytI
UniProt ID: (Bovine) P48018, (Rat) P21707, (Mouse) P46096
Entrez Gene ID: (Bovine) 281511, (Rat) 25716, (Mouse) 20979
calmodulin binding
SNARE binding
phosphatidylserine binding
transporter activity
calcium ion binding
protein binding
phospholipid binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
protein C-terminus binding
syntaxin-1 binding
syntaxin binding
clathrin binding
identical protein binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
metal ion binding
membrane trafficking regulatory protein
cell differentiation
positive regulation of calcium ion-dependent exocytosis of neurotransmitter
detection of calcium ion
neurotransmitter secretion
synaptic vesicle exocytosis
calcium ion regulated exocytosis
regulation of calcium ion-dependent exocytosis
positive regulation of vesicle fusion
positive regulation of calcium ion-dependent exocytosis
vesicle docking
positive regulation of synaptic transmission
response to calcium ion
regulation of synaptic transmission, glutamatergic
fast, calcium ion-dependent exocytosis of neurotransmitter
regulation of regulated secretory pathway
synaptic vesicle endocytosis
positive regulation of dendrite extension
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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